[Histonet] OT then freeze?

[renton louise mrs]

Talk about showing age: I used to use a freezing microtome for in-theatre frozen sections. It was housed in a little cubicle just off the recovery room. What those poor people thought of the deafening WHOOSH of the Co2 I never found out!
Regards

-----Original Message-----
From: "Houston, Ronnie" <Ronnie_Houston <@t> bshsi.com>
To: "'KMIN (Kathy Mink)'" <minkk <@t> zgi.com>, histonet <@t> lists.utsouthwestern.edu
Date: Tue, 13 Jul 2004 09:58:37 -0400
Subject: RE: [Histonet] Formalin fix---then freeze?


Absolutely; although there is a very real possibility that the tissue will
float off the slide (no matter what type of slide you use) if cutting with a
cryostat. Wash the tissue well before freezing. 
The microtome of choice for sectioning frozen fixed tissue is a freezing
microtome, although there aren't many about any more. What a shame!....now
that is an art and experience that younger histotechs are losing out on....I
remember the days of free-floating cerebellum
sections................showing my age now.

Ronnie

Ronnie Houston
Director of Anatomic Pathology
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 2877972
(804) 2877906 - fax
ronnie_houston <@t> bshsi.com

  



-----Original Message-----
From: KMIN (Kathy Mink) [mailto:minkk <@t> zgi.com]
Sent: Monday, July 12, 2004 8:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Formalin fix---then freeze?


Hi,

I have been asked an unusual (stupid?) question and hope someone can help.
Is it possible to formalin fix a tissue and then put it in OCT and freeze it
to cut frozen sections?  I know it doesn't make much sense but apparently a
contract we have was worded this way (obviously not proof read by the
pathologist) and now I need to treat the tissue this way if possible.  Help!

Kathy Mink


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Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
.......so what IS the speed of dark?