[Histonet] Formalin fix---then freeze?

[Tony Henwood]

Gudrun,

Yes, we have often had to resort to this. 
Rinse the tissue in water 30min.
Infiltrate in 30% OCT (room temp)or sucrose  untill the tissue sinks.
Pat the tiisue to remove excess fluid
Embed in OCT, freeze and section
Use sticky slides to  help adherence.
I usually air dry for 15-30 min to aid adhesion
Stain for Fats etc as required.


Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318



-----Original Message-----
From: Gudrun Lang [mailto:gu.lang <@t> gmx.at] 
Sent: Wednesday, 14 July 2004 12:58 AM
To: Histonetliste
Subject: Re: [Histonet] Formalin fix---then freeze?


Would this method be adequate for lung to perform Sudan-stain on it?
That opens the possibility to store the autopsy-tissue in formalin and do
the staining later.
Has anybody experience with this?

Gudrun Lang

----- Original Message -----
From: "KMIN (Kathy Mink)" <minkk <@t> zgi.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, July 13, 2004 2:09 AM
Subject: [Histonet] Formalin fix---then freeze?


Hi,

I have been asked an unusual (stupid?) question and hope someone can help.
Is it possible to formalin fix a tissue and then put it in OCT and freeze it
to cut frozen sections?  I know it doesn't make much sense but apparently a
contract we have was worded this way (obviously not proof read by the
pathologist) and now I need to treat the tissue this way if possible.  Help!

Kathy Mink


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