[Histonet] Formalin fix---then freeze?

[Smith, Allen]

This used to be standard operating procedure for studies of phosphatases and
cholinesterase. Several such protocols are in the 2nd and 3rd editions of
Pearse's HISTOCHEMISTRY: THEORETICAL AND APPLIED.  (2nd and 3rd editions are
available used.  The 4th edition is unobtainable.)

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University
School of Graduate Medical Sciences
            Podiatric Medicine and Surgery
Miami Shores, Florida


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of KMIN (Kathy
Mink)
Sent: Monday, July 12, 2004 8:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Formalin fix---then freeze?



Hi,

I have been asked an unusual (stupid?) question and hope someone can help.
Is it possible to formalin fix a tissue and then put it in OCT and freeze it
to cut frozen sections?  I know it doesn't make much sense but apparently a
contract we have was worded this way (obviously not proof read by the
pathologist) and now I need to treat the tissue this way if possible.  Help!

Kathy Mink


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