[Histonet] Formalin fix---then freeze?

[Hermina Borgerink]

We routinely do lipid staining on formalin fixed archival tissue and use the following procedure:  wash the tissue specimen under running water for an hour to remove the formalin.  Then infiltrate overnight at 4°C with 15% sucrose in PBS.  Freeze the tissue in OCT and section at 5 - 6 µm. We use Churukian's Oil Red O as our fat stain.  By cryoprotecting the tissue in sucrose, the morphology is comparable to that of a paraffin section. 
Hermina


Hermina M. Borgerink, BA, HTL(ASCP)QIHC

Wake Forest University Health Sciences
Department of Pathology
Medical Center Blvd.
Winston-Salem, NC 27157
Tel. (336) 716-1538
Fax (336) 716-1515
e-mail hborgeri <@t> wfubmc.edu
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Tuesday, July 13, 2004 10:58 AM
To: Histonetliste
Subject: Re: [Histonet] Formalin fix---then freeze?

Would this method be adequate for lung to perform Sudan-stain on it?
That opens the possibility to store the autopsy-tissue in formalin and do
the staining later.
Has anybody experience with this?

Gudrun Lang

----- Original Message -----
From: "KMIN (Kathy Mink)" <minkk <@t> zgi.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, July 13, 2004 2:09 AM
Subject: [Histonet] Formalin fix---then freeze?


Hi,

I have been asked an unusual (stupid?) question and hope someone can help.
Is it possible to formalin fix a tissue and then put it in OCT and freeze it
to cut frozen sections?  I know it doesn't make much sense but apparently a
contract we have was worded this way (obviously not proof read by the
pathologist) and now I need to treat the tissue this way if possible.  Help!

Kathy Mink


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet