FW: [Histonet] Dezenkerizing slides

[Carole Fields]

oops..I forgot to send it to the HistoNet.
CF

-----Original Message-----
From: Carole Fields 
Sent: Monday, July 12, 2004 2:55 PM
To: 'Gayle Callis'
Subject: RE: [Histonet] Dezenkerizing slides


It is in Ann Preese, us old-timers remember.  0.5% Alc-Iodine.  It is the
only place I could find it.  We use it every day.

 5 gms     Iodine
1000ml    80% alcoh
Dissolve Iodine in 10 to 20 ml of the 80% alcoh.  When dissolved add the
remainder of the alcohol.  We run our slides down on the stainer to 95% alc
then they go into Alc-Iodine for 6-8 min. then wash and finish staining H&E.
The Iodine washes out and you are done.  You do not need the hypo.

Carole Fields
Lex Med Ctn
W.Columbia, SC

-----Original Message-----
From: Gayle Callis [mailto:gcallis <@t> montana.edu]
Sent: Monday, July 12, 2004 2:31 PM
To: Cheryl Crowder; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Dezenkerizing slides


Hi Cheryl and all, 

This answer has come up twice, but what is the percentage of iodine in the
70%alcohol IF one can't locate it in a book, etc? 


>Ben - Years ago, when Zenker's was used by everyone, we used to put 
>alcoholic iodine as the first station on the processor (after formalin).  
>Then we added a sodium thiosulfate rinse to the stain routine.  That took
an 
>extra minute or 2.   You might try that.  Cheryl
>
>Cheryl Crowder, BA, HTL(ASCP)
>Chief Technologist
>Anatomic Pathology
>Department of Pathobiological Sciences
>School of Veterinary Medicine
>Louisiana State University
>Skip Bertman Drive
>Baton Rouge, LA  70803
>
>225-578-9734
>FAX:  225-578-9720
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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