[BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER

lpwenk <@t> sbcglobal.net lpwenk <@t> sbcglobal.net
Sat Jul 10 04:57:51 CDT 2004


Reminder to everyone who is dezenkerizing (lugolizing) on the stainers -
when you get ready to dump this iodine station, it cannot go down the sink.
It now contains mercuric salts, so must be collected as mercury waste. (As
should the next couple of containers of reagents, carry-over on the slides.)

Ways to get around this:
1. Dezenkerize (lugolize) by hand, then add the slide to the stainer.
2. Switch over to a zinc fixative.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

----- Original Message -----
From: "Fred Underwood" <funderwood <@t> mcohio.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, July 09, 2004 3:36 PM
Subject: Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER


> Set up a reagent station with idodine, 5 minutes shoud do.  From there
> just go through your rehydrating alcohols, tap water wash, and continue
> your staining procedure.
>
> Fred
>
> >>> "Shelkowsky, Ben" <Ben.Shelkowsky <@t> chomp.org> 07/09/04 03:21PM >>>
> I would like to dezenkerize my slides from mercury-fixed tissues on the
> SAKURA automated stainer in a program I can internalize on the menu. Is
> anyone out there already doing this and can you send me your procedure?
>
> We would appreciate any help on this matter.
>
> Ben Shelkowsky
> CHOMP
> Monterey, CA. 93950
> ben.shelkowsky <@t> chomp.org
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