[Histonet] Antigen retrieval - Microwave problem

[SMITH,REBEKAH FELICIA]

Hi Jaime! I can definitely sympathize. I was having the same 
problem with slides I'm using for immunohistochemistry (subbed 
with poly-l-lysine, and I put gelatin in the waterbath). What I 
ended up doing is taking buffer in a coplin jar (i use 8.5 pH .5M 
tris) putting it in the microwave, and measuring the temperature 
in the jar every 10 sec. Tedious I know, but it gave me a good 
idea of how long it takes to boil and how hot it is when it is 
boiling, so I was able to choose a time that was just before the 
boiling point. I put my slides in a coplin jar in a small water 
bath in the microwave for 45 sec and then repeat emptying and 
refilling the water. Good luck!
--
SMITH,REBEKAH FELICIA
"You are a child of the universe, no less than the trees and the 
stars
You have a right to be here and whether or not it is clear to you, 
no doubt the universe is unfolding as it should. Therefore be at 
peace with G-d, whatever you conceive Him to be. And whatever your 
labors and aspirations,in the noisy confusion of life, keep peace 
in your soul.-Max Ehrmann,"Desiderata"