[Histonet] nissl stain

Smith, Allen asmith <@t> mail.barry.edu
Tue Jul 6 20:00:48 CDT 2004


A good Nissl stain stains only RNA, but some "Nissl stains" also stain DNA.
It's easy to tell the Nissl bodies from the nuclei, because they are smaller
and irregular.  Since Nissl bodies have high concentrations of RNA, they
stain darkly.  Nissl bodies are characteristic of neurons with long axons.
Since neurons with short axons and glial cells have low concentrations of
RNA, they stain weakly, if at all.  

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University
School of Graduate Medical Sciences
            Podiatric Medicine and Surgery
Miami Shores, Florida


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
mgorin <@t> umich.edu
Sent: Tuesday, July 06, 2004 12:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] nissl stain



sry guys i am new at this.  but can somone tell me if nissl, when doen to
brain
tissue, will only stain neurons?  will it stain glial cells?

also, how does nissl work. wat bodies does it stain.

thanks.

-mike-
UMICH- Ann Arbor


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Barry University - Miami Shores, FL (http://www.barry.edu) 




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