[Histonet] fixing and processing - testicular biopsy

[Gudrun Lang]

Dear histonetters
Our usual treatment of testicular biopsies is:
fixing a few hours in bouin's solution, then putting them together with all other biopsies in the VIP. 
processing begins with NFB-70-80-clearing-paraffin, over night.

1. question:
Does this treatment make sense at all? Is it estimated, that the result would be the same with or without bouin?
2. please let me know about a proper processing-protocol for testicular biopsies. (needle-biopsies, VIP)
3. Do you know any good substitute for bouin, that does'nt effect Immunhisto for this purpose?

many thanks
Gudrun Lang
Akh Linz, Austria