[Histonet] fixing and processing testicular biops.

[Gudrun Lang]

Dear Tim,
I've read controvers texts. On one side John Kiernan writes, that is does
not make sense to put bouin-fixed tissue in NFB. (because  it solutes the
picrates?)
On the other side I read, that the tissue should be washed in water or
Lithiumcarbonatsolution.
My problem is, if we should create a proper protocol for our biospies or if
the old one is good enough.

Gudrun Lang


----- Original Message -----
From: "Morken, Tim - Labvision" <tpmorken <@t> labvision.com>
To: "'Gudrun Lang'" <gu.lang <@t> gmx.at>
Sent: Tuesday, July 06, 2004 8:50 PM
Subject: RE: [Histonet] fixing and processing testicular biops.


> Gudrun, Bouin's is the preferred fixative for testis. And Bouin's is
> generally an excellent fixative for immunohistochemistry. Normally,
bouin's
> - fixed tissue should be rinsed in water for 30 minutes after fixation and
> before further processing
>
> Tim Morken
> Lab Vision - Neomarkers
> www.labvision.com
>
> Free webhosting for US State Histotechnology Societies:
> http://www.labvisioncorp.com/demowebsite/index.cfm
>
> -----Original Message-----
> From: Gudrun Lang [mailto:gu.lang <@t> gmx.at]
> Sent: Tuesday, July 06, 2004 11:35 AM
> To: Histonetliste
> Subject: [Histonet] fixing and processing testicular biops.
>
>
> Dear histonetters
> Our usual treatment of testicular biopsies is:
> fixing a few hours in bouin's solution, then putting them together with
all
> other biopsies in the VIP.
> processing begins with NFB-70-80-clearing-paraffin, over night.
>
> 1. question:
> Does this treatment make sense at all? Is it estimated, that the result
> would be the same with or without bouin? 2. please let me know about a
> proper processing-protocol for testicular biopsies. (needle-biopsies, VIP)
> 3. Do you know any good substitute for bouin, that does'nt effect
Immunhisto
> for this purpose?
>
> many thanks
> Gudrun Lang
> Akh Linz, Austria _______________________________________________
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