[Histonet] Von Kossa staining

[Caroline Stott]

Hi Tiffany,
I have used this procedure many a time.
If you need a control, immerse one slide in 4.5% citrate buffer for 30-40 
minutes.
Wash all slides in distilled water
Flood slides with 5% silver nitrate
Expose to bright sunlight, or UV light for 20 minutes, or a 60-watt lamp 
(about 4-5 inches from the slides) for 30-60 minutes
Wash in several changes of distilled water
Flood slides with 5% sodium thiosulphate for 2-3 minutes
Counterstain with neutral red, safranine or van Gieson.
Clear and mount.
Hope that helps
Caroline

Caroline Stott

Histology Service Unit
University of Otago
PO Box 913
Dunedin, New Zealand
Ph  (03) 479 7152
Fax (03) 479 7136