[Histonet] Von Kossa staining

[Yaskovich, Ruth A (NIH/NIDCR)]

Tiffany,
I have done Von-Kossa's on MMA thick about 200 microns or more specimens
whole not mounted on slides. Didn't remove any plastic just dropped the
specimens right into the silver solution and into a window in the sun. Got
wonderful positive results I would run a test slide on the GMA slide first
and see what you get. Good luck!
Ruth Yaskovich
National Institutes of Health
National Institute of Dental and Crainiofacial Research
Neuronal Gene Expression Section
> ----------
> From: 	Tiffany L Sheffield
> Sent: 	Thursday, July 1, 2004 9:16 AM
> To: 	histonet <@t> lists.utsouthwestern.edu
> Subject: 	[Histonet] Von Kossa staining
> 
> <<File: ATT833166.txt>>
> Hello Fellow Histonetters!
> 
>  I have a quick question. I need to do a Von Kossa stain on some artery
> vessels with calcium deposits present. I have embedded them in GMA not
> MMA. I have a modified procedure for  bone to detect for calcium
> deposits but  you remove the plastic. If anyone has a protocol they
> would like to share for GMA it would be appreciated. Also, if anyone
> knows of any other stains for GMA for calcium deposit detection it would
> be greatly appreciated. Oh, I almost forgot the polymer I am using is
> Technovit 7200.
> 
> Thank you,
>  Tiffany
> 
> 

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