[Histonet] Re: Histonet Digest, Vol 7, Issue 92

Thu Jul 1 10:17:27 CDT 2004

----- Original Message ----- 
From: <histonet-request <@t> lists.utsouthwestern.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, June 30, 2004 9:22 AM
Subject: Histonet Digest, Vol 7, Issue 92

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> Today's Topics:
>
>    1. 2 questions (Jennifer Sipes)
>    2. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels)
>    3. Guinea pig mast cells: (tony.j.savage <@t> gsk.com)
>    4. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels)
>    5. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels)
>    6. Job Opportunities in Histology  Latest Update 06/30/04
>       (Pam Barker (extension 234))
>    7. Florida License (Terry Murphy)
>    8. Cleaning Metal Cassettes (Billie Zimmerman)
>    9. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels)
>   10. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels)
>   11. unscubscribe (Marnie Whiteside)
>   12. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels)
>   13. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels)
>   14. Re: New immunostainer (CrochiereSteve <@t> aol.com)
>   15. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels)
>   16. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 30 Jun 2004 05:02:20 -0700 (PDT)
> From: Jennifer Sipes <jengirl1014 <@t> yahoo.com>
> Subject: [Histonet] 2 questions
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20040630120220.2710.qmail <@t> web60609.mail.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> My name is Jennifer.  I have recently taken on the task of doing histology
for my lab.  I have just 2 questions:
>
> 1.  Does anyone have a protocol for Toludine Blue staining?
>
> 2.  A girl in my lab is cryo fixing monkey lung.  The procedure goes as
follows:
>           Each lung is sectioned into 9 parts.  Of the total 18 lung
sections, only 5% of
>           them go for cryosectioning.  They are interested in the the
large airways.
>      She is having a problem with getting decent cuts.  Currently, she is
submerging the
>      small pieces into OCT overnight at 4 C  then mounting them.  Does
anyone happen
>      to have a protocol better than that.  I told her that that was the
incorrest way, but she
>      said that was the protocol she was given.  Please remember that the
rest of the
>      tissue is going for Real Time PCR, RNA extraction, etc.
>
> I would greatly appreciate any information that you folks could give me.
Thnak you for your time.
>
>
>
>
> Jennifer K. Sipes
> Sr. Laboratory Technician
> Johns Hopkins University
> Ross Research Building Rm 929
> 720 Rutland Avenue
> Baltimore, MD  21205
> phone:  410-614-0131
> cell:     443-413-0853
> fax:     410-955-9677
> e-mail:  jengirl1014 <@t> yahoo.com
>
> Jennifer;
    Here is a Toluidine Blue protocol. The first is an aqueous mount, the
second can be mounted in a resin.
Solution'\;
Toluidine blue 0.5% in 20% ethanol

1. Deparaffinize to water.
2. Toluidine blue 5 to 20 minutes.
3. Wash briefly in tap water
4.Coverslip from water.
These slides are  not permanent.

1.Follow first three steps as above
2.Then two changes each of
 95%, Absolute, Xylene
each one minute.
Mount in Permount
Hope this helps
                          Thelma

>
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> ------------------------------
>
> Message: 2
> Date: Wed, 30 Jun 2004 03:11:54 -0500
> From: Kathy Abels <kabels <@t> sial.com>
> Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office.
> To: histonet <@t> lists.utsouthwestern.edu
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>
> I will be out of the office starting  06/30/2004 and will not return until
> 07/12/2004.
>
> I will respond to your message when I return.
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> ------------------------------
>
> Message: 3
> Date: Wed, 30 Jun 2004 10:51:20 +0100
> From: tony.j.savage <@t> gsk.com
> Subject: [Histonet] Guinea pig mast cells:
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <OF529B847C.E16A0DC9-ON80256EC3.00359AA9 <@t> sb.com>
> Content-Type: text/plain; charset=us-ascii
>
> My group is trying to find a good method for demonstrating guinea pig mast
> cells which appear morphologically different to human and rodent mast
> cells. I have been unable to find an antibody which demonstrates guinea
> pig mast cells and the metachromatic tinctorial methods show only weak
> staining. Does anyone have a good method to share with us for
> demonstrating guinea pig mast cells, tinctorially or
> immunohistochemically?
>                                        Kind Regards,
>                                                     Tony Savage
> Histopathology Group
> Asthma Biology Department.
> RIRP CEDD.
> GlaxoSmithKline Medicines Research Centre,
> Gunnelswood Road,
> STEVENAGE,
> Hertfordshire.
>   SG1 2NY
> tel.          +44 (0)1438 764117
> fax.         +44 (0)1438 764782
> email.    Tony.J.Savage <@t> gsk.com
> mobile    +44 07753609835
> http://ukdiscovery.gsk.com/histopathology/default.htm
>
>
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> ------------------------------
>
> Message: 4
> Date: Wed, 30 Jun 2004 06:43:54 -0500
> From: Kathy Abels <kabels <@t> sial.com>
> Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office.
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OF4E30EDB9.9EFEC568-ON86256EC3.004071BC-86256EC3.004071BC <@t> sial.com>
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>
> I will be out of the office starting  06/30/2004 and will not return until
> 07/12/2004.
>
> I will respond to your message when I return.
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> ------------------------------
>
> Message: 5
> Date: Wed, 30 Jun 2004 01:35:54 -0500
> From: Kathy Abels <kabels <@t> sial.com>
> Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office.
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OFEA96C060.FE8CDD22-ON86256EC3.00243F0A-86256EC3.00243F0A <@t> sial.com>
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> I will be out of the office starting  06/30/2004 and will not return until
> 07/12/2004.
>
> I will respond to your message when I return.
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> ------------------------------
>
> Message: 6
> Date: Tue, 29 Jun 2004 21:01:18 -0400
> From: Pam Barker (extension 234) <pam <@t> ategra.com>
> Subject: [Histonet] Job Opportunities in Histology  Latest Update
> 06/30/04
> To: Histonetters <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <E1BfTS9-0003th-00 <@t> cardinal.mail.pas.earthlink.net>
> Content-Type: Text/Plain
>
> Hi Histonetters, I hope you have a Happy 4th of July.
>
> Here is the latest update on opportunities with some of my best clients
throughout the US who are seeking Histology Supervisors, Histo Technologists
and Histo Technicians.  These are positions as direct employees of our
client. There are fulltime 40 hour per week positions.. As a direct employee
of one of our clients you will be provided with full benefits including
Health Insurance, Vacation, Sick Pay, Relocation money and a lucrative
sign-on bonus.  I have part time and temporary positions as well.
>
> I have supervisory, team lead and bench positions.  These positions
require HTL or HT certification or registry eligibility.
>
> Here are some of my HOTTEST Histo Tech and Histology Supervisor positions:
> 1.  Tampa Bay, FL Area - MOHS Tech
> 2.  Ft. Lauderdale, FL - Histo Tech
> 3.  North Central Georgia - Histo Tech
> 4.  Central Pennsylvania - Histo Tech multiple openings
> 5.  Western Pennsylvania - Histo Tech
> 6.  Central Alabama -  Histology Supervisor
> 7.  Southern Arizona - Histo Tech multiple openings and locations
> 8.  Southern Maine - Immunohistochemistry Tech
> 9.  Southern Maine - Histo Tech
> 10. Northern California - Histo Tech (1 full time and 1 part time)
> 11. Western Missouri - Histo Tech
> 12. Eastern North Carolina - Histo Tech
> 13. Central Virginia - Histo Tech (TEMP)
>
> If you are interested in these jobs, please CALL ME ASAP at 800 466 9919
x234. To speed things up, please also send me a copy of your resume, (if you
haven't already done so). If you are interested in jobs outside the
above-mentioned areas, please send me your resume as well. I have clients
throughout the US. I will keep your resume confidential and will not release
it to anyone without your permission (This is Ategra policy as well as my
own). My services are at no charge to you.
>
> Of course, you may be happy in your present job, but it never hurts to to
keep an eye open. Also, if you have friends/peers who might be interested as
well,  if you could pass my query & name on to them I'd be very grateful.
>
> However, if you are interested in any of the jobs above, please call me.
>
> Happy 4th of July!!
>
> Pam - 800 466 9919 ext 234
>
> ---------------------------------------------------------
> Ategra Systems Inc
> Specialists in Permanent & Contract Staffing
>
> Learn More About Ategra:
> <http://www.ategra.com/service/service.html>
>
> Pam Barker
> Senior Lab Recruiter
> Ategra Systems Inc
> Specialists in Permanent & Contract Staffing
> 7085 University Blvd.
> Winter Park, FL 32792
>
> VOICE:    407-671-5800 ext 234
> TOLLFREE: 800-466-9919 ext 234
> EMAIL: pam <@t> ategra.com <mailto:pam <@t> ategra.com>
>
> To Learn More About Ategra:
> http://www.ategra.com
> --------------------------------------------------------------------------
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> ------------------------------
>
> Message: 7
> Date: Tue, 29 Jun 2004 16:10:08 -0700 (PDT)
> From: Terry Murphy <cbuddyh <@t> yahoo.com>
> Subject: [Histonet] Florida License
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20040629231008.88913.qmail <@t> web52304.mail.yahoo.com>
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> Hi.
>
> I am working as a traveling temporary Histotech.  My temp agency has an
opportunity in FL and I do not have a FL License.  Does anyone know if there
is a way to obtain a temporary FL License?
>
> Thanks
>
> Terry Murphy
>
>
> ---------------------------------
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> ------------------------------
>
> Message: 8
> Date: Tue, 29 Jun 2004 14:49:45 -0400
> From: "Billie Zimmerman" <bzimmerm <@t> mail.mcg.edu>
> Subject: [Histonet] Cleaning Metal Cassettes
> To: <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <s0e181a0.083 <@t> mail.mcg.edu>
> Content-Type: text/plain; charset=US-ASCII
>
> I'd like to know the procedure for cleaning metal cassettes.
> Thanks,
> Billie Zimmerman
> Medical College of GA
>
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> ------------------------------
>
> Message: 9
> Date: Wed, 30 Jun 2004 00:22:53 -0500
> From: Kathy Abels <kabels <@t> sial.com>
> Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office.
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OF89F31F11.AEC8EA74-ON86256EC3.001D8FD5-86256EC3.001D8FD5 <@t> sial.com>
> Content-Type: text/plain; charset=US-ASCII
>
>
>
>
>
> I will be out of the office starting  06/30/2004 and will not return until
> 07/12/2004.
>
> I will respond to your message when I return.
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>
>
> ------------------------------
>
> Message: 10
> Date: Wed, 30 Jun 2004 05:38:53 -0500
> From: Kathy Abels <kabels <@t> sial.com>
> Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office.
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OFEACF62C1.38B8DB6F-ON86256EC3.003A7E0B-86256EC3.003A7E0B <@t> sial.com>
> Content-Type: text/plain; charset=US-ASCII
>
>
>
>
>
> I will be out of the office starting  06/30/2004 and will not return until
> 07/12/2004.
>
> I will respond to your message when I return.
> _______________________________________________
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>
>
> ------------------------------
>
> Message: 11
> Date: Wed, 30 Jun 2004 07:00:47 -0400
> From: "Marnie Whiteside" <mwhitesi <@t> adventisthealthcare.com>
> Subject: [Histonet] unscubscribe
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <s0e2653c.049 <@t> GWGATE1.ahm.com>
>
>
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>
> ------------------------------
>
> Message: 12
> Date: Wed, 30 Jun 2004 01:35:54 -0500
> From: Kathy Abels <kabels <@t> sial.com>
> Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office.
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OFEA96C060.FE8CDD22-ON86256EC3.00243F0A-86256EC3.00243F0A <@t> sial.com>
> Content-Type: text/plain; charset=US-ASCII
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>
>
>
>
> I will be out of the office starting  06/30/2004 and will not return until
> 07/12/2004.
>
> I will respond to your message when I return.
> _______________________________________________
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>
>
> ------------------------------
>
> Message: 13
> Date: Wed, 30 Jun 2004 02:53:53 -0500
> From: Kathy Abels <kabels <@t> sial.com>
> Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office.
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OF6EC33968.8F5E63F4-ON86256EC3.002B62FB-86256EC3.002B62FB <@t> sial.com>
> Content-Type: text/plain; charset=US-ASCII
>
>
>
>
>
> I will be out of the office starting  06/30/2004 and will not return until
> 07/12/2004.
>
> I will respond to your message when I return.
> _______________________________________________
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>
>
> ------------------------------
>
> Message: 14
> Date: Tue, 29 Jun 2004 21:41:06 EDT
> From: CrochiereSteve <@t> aol.com
> Subject: Re: [Histonet] New immunostainer
> To: histo <@t> sdspathology.com.au, histonet <@t> lists.utsouthwestern.edu
> Message-ID: <147.2d469ff0.2e137432 <@t> aol.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> I'm quite happy with my new Nemesis from Biocare Medical.
> It has the highest slide and reagent capacity that I could find and the
> reagents are much less expensive than the othe company's which I had been
using.
>
>
>
> Steven M. Crochiere, HT(ASCP)
> Histology Supervisor
> LifePath Partners @ Mercy Medical Center
> Springfield, MA 01104
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>
> ------------------------------
>
> Message: 15
> Date: Wed, 30 Jun 2004 01:05:54 -0500
> From: Kathy Abels <kabels <@t> sial.com>
> Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office.
> To: histonet <@t> lists.utsouthwestern.edu
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> <OF977AB2C5.46396DD7-ON86256EC3.00217FD9-86256EC3.00217FD9 <@t> sial.com>
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> I will be out of the office starting  06/30/2004 and will not return until
> 07/12/2004.
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> I will respond to your message when I return.
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> ------------------------------
>
> Message: 16
> Date: Wed, 30 Jun 2004 02:36:54 -0500
> From: Kathy Abels <kabels <@t> sial.com>
> Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office.
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OF64267423.4CB64BAC-ON86256EC3.0029D4D2-86256EC3.0029D4D2 <@t> sial.com>
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> I will be out of the office starting  06/30/2004 and will not return until
> 07/12/2004.
>
> I will respond to your message when I return.
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> End of Histonet Digest, Vol 7, Issue 92
> ***************************************