[Histonet] Hematoxylin

[Gudrun Lang]

George,
thanks for your advice. Yes, we belong to this old fashioned labs, that mix
their stain solutions by themeselves.
We tried the purchaseable hematoxilines, but our pathologiest did not like
any change. Because of the steady exchange of solutions, there is a constant
colouring. The hematoxilin bottles are used up within a month. It is a kind
of question of believe, I think.
greetings
Gudrun


----- Original Message -----
From: "George Cole" <georgecole <@t> ev1.net>
To: "'Gudrun Lang'" <gudrun.lang <@t> aon.at>
Sent: Friday, January 30, 2004 12:25 AM
Subject: RE: [Histonet] Hematoxylin


> Gudrun
> If you can find a source, GILL's III would replace all three of your
> hematoxylins. It keeps so well, you might well be able to order enough
> from Sigma or some other supplier to last for months. Mayer's, remember,
> lasts only 30 days after being mixed. It does not stain steadily for 30
> days then die---it starts fading day by day from the beginning, thus
> bringing in some variation you don't want.  Harris demands clarification
> with acid alcohol, thus letting in variations between those who do the
> dunking in the acid alcohol, and Weigert's---I will make no comment on
> Weigert's---I never used it---I simply saw the Weigert mixtures dating
> from the 1900's.  If it compares favorably with the Gill's III I will
> swim from here to Linz without my water wings!!!
> georgecole <@t> ev1.net
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun
> Lang
> Sent: Thursday, January 29, 2004 10:27 AM
> To: Histonetliste; mark.lewis <@t> thermo.com
> Subject: Re: [Histonet] Hematoxylin
>
> We use Mayer's hematoxylin for routine HE, and Harris hematoxylin for
> cryocuts.
> Weigert is used with specistains.
>
> Gudrun Lang
> general hospital, Linz, Austria
>
> ----- Original Message -----
> From: <mark.lewis <@t> thermo.com>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Thursday, January 29, 2004 4:31 PM
> Subject: [Histonet] Hematoxylin
>
>
> > Hello everyone !
> >
> > I'm taking a survey to find out which type of Hematoxylin is most
> commonly
> > used in Histology and Cytology labs.
> >
> > Thanks !
> >
> > Best regards,
> >
> > Mark
> >
> > Mark Lewis
> > Product Specialist
> > Anatomical Pathology
> > Clinical Diagnostics
> > Thermo Electron Corporation
> > (412) 747-4013
> > (412) 788-1097
> > E-mail: mark.lewis <@t> thermo.com
> >
> >
> >
> >
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