[Histonet] Hematoxylin

[Caroline Stott]

Hi Mark,
We use
5g Haematoxylin
100g Ammonium Alum
50ml Ethyl alcohol
1000ml Distilled water
0.37g Sodium iodate
  and when cool we add 40ml glacial acetic acid.  So it is acidified.

We use weak acid alcohol to differentiate the slides, followed by an 
alcoholic eosin Y.
Good luck with the survey
Caroline


Caroline Stott

Histology Service Unit
Medical School
University of Otago
Dunedin
(03) 479 7152