[Histonet] phospho-specific antibodies

[Sennello, Gina]

I was wondering how everyone was fixing tissue (xenograft tumors) for
detection of phospho-specific antibodies such as pcKit, pAKT, pKDR and
others?  Has anyone found that cold fixatives stop the  de-phosphorylation
process better than room temp?  We have seen a staining pattern that
suggests that something is going on with either the penetration of the
fixative or the phosphorylation process upon removal of the tumor.  Any
suggestions would be welcome.
Thanks in advance,

Gina Sennello
Associate Scientist/ Histotechnologist

OSIP
2860 Wilderness Place
Boulder, CO
80301

phone 303-546-7739
fax      303-444-0672