[Histonet] Muscle fiber differentiation

[Andi Kappeler]

Hi Sue

We routinely use the following mouse monoclonal antibodies on FFPE human
skeletal muscle:
Myosin I (slow), clone NOQ7.5.4D, Sigma M-8421; working conc. 5 ug Ig/ml,
HIER in 10 mM citrate buffer, pH 6.0, pressure cooker.
Myosin II (fast), clone MY-31, Sigma M.4276; working conc. 10 ug Ig/ml, HIER
as above.
Visualization is with a StrABC system.
Hope this helps.

Andi Kappeler
Insitute of Pathology, University of Bern, Switzerland


----- Original Message ----- 
From: "Susan Q Wells" <susan.wells <@t> bms.com>
To: <histonet <@t> pathology.swmed.edu>
Sent: Wednesday, January 28, 2004 2:00 PM
Subject: [Histonet] Muscle fiber differentiation


> Does anyone have any ideas on how to distinguish Type 1 and Type
> 2 muscle fibers with IHC? We have done  an ATPase  stain but
> wanted to know if there are available antibodies?
> Thanks in advance for your help,
> Sue Wells HT(ASCP),QIHC
>
>
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