[Histonet] staining for stellate cells

[Edwards, R.E.]

.....and  if  you  eat  polar  bear  liver, the  vitamin  A  will  kill  you!
                                                                             E.S. Kimo
                                                                              

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Marshall
Terry Dr,Consultant Histopathologist
Sent: 28 January 2004 16:08
To: Geoff McAuliffe; Michele Ellender
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] staining for stellate cells


No, I think the stellate cell is the lipocyte, or cell of Ito, which lies within the space of Disse.
I know of nothing but a fat stain to demonstrate, and as always that would show the fat not the cell.

For everyone's information, they become enlarged (and store the vitamin) in Vitamin A poisoning.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
 England
        terry.marshall <@t> rothgen.nhs.uk

-----Original Message-----
From: Geoff McAuliffe [mailto:mcauliff <@t> umdnj.edu]
Sent: 28 January 2004 15:40
To: Michele Ellender
Cc: 'histonet <@t> lists.utsouthwestern.edu'
Subject: Re: [Histonet] staining for stellate cells


    By stellate cells do you mean Kupffer cells, the fixed macrophages 
in the sinusoids? If so, any antibody that stains macrophages will work. 
For mouse Mac1 or F4/80 will work on frozen sections of fixed tissue.

Geoff

Michele Ellender wrote:

>Hello all,
>does anyone have a staining method to stain for stellate cells in mouse
>liver sections?
>Thanks,
>Michele 
>
>Dr Michele Ellender
>Radiation Effects Department,
>NRPB, Chilton, Didcot, 
>Oxon. OX11 0RQ.  UK
>
>michele.ellender <@t> nrpb.org
>
>
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-- 
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**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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