[Histonet] cryostat sections of undecalcified tissue implants

[Alan Bright]

Dear Elizabeth,

Our cryostats fitted with the 5030 & 5040 have no problem sectioning the
bone you mention, using a standard C profile Tungsten carbide tipped
knife and temperature settings for the cryochamber @ -28 to -30ºC. By
using a C profile knife the bone is delivered onto the knife face
through the standard anti-roll system without the stress effect caused
by D profile knives, which also disposes of the need for the costly and
slow, tape transfer method.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright <@t> brightinstruments.com
Web Site: www.brightinstruments.com



-----Original Message-----
From: Elizabeth Chlipala [mailto:lizchlipala <@t> premierhistology.com] 
Sent: 26 January 2004 19:18
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cryostat sections of undecalcified tissue implants


 
Hello all
 
Is it possible to section undecalcified cryostat sections from implants
(no plastic) from a rat subcutaneous implant animal model.  The implants
will have possibly new bone and cartilage, not very dense bone, but some
bone.  Would I need to use a tape transfer system or could I use a
tungsten knife and be o.k.  Any suggestions would be appreciated.
 
Thanks in advance
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
lizchlipala <@t> premierhistology.com
www.premierhistology.com
 
Ship to Address:
Premier Histology Laboratory
University of Colorado
MCBD, Room A3B40
Boulder, Colorado 80309
 
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