[Histonet] cryostat sections of undecalcified tissue implants

[Gayle Callis]

Tape transfer but try TC knife first, bone will give you the most problem
but you may survive with a really sharp, new edge. You will have to set the
temperature cold, -28C to -30C usually works.  


At 12:17 PM 1/26/2004 -0700, you wrote:
> 
>Hello all
> 
>Is it possible to section undecalcified cryostat sections from implants
>(no plastic) from a rat subcutaneous implant animal model.  The implants
>will have possibly new bone and cartilage, not very dense bone, but some
>bone.  Would I need to use a tape transfer system or could I use a
>tungsten knife and be o.k.  Any suggestions would be appreciated.
> 
>Thanks in advance
> 
>Liz
> 
>Elizabeth A. Chlipala, BS, HTL(ASCP)
>Premier Histology Laboratory, LLC
>P.O. Box 18592 
>Boulder, Colorado 80308
>Office: (303) 735-5001
>Fax: (303) 735-3540
>lizchlipala <@t> premierhistology.com
>www.premierhistology.com
> 
>Ship to Address:
>Premier Histology Laboratory
>University of Colorado
>MCBD, Room A3B40
>Boulder, Colorado 80309
> 
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)