[Histonet] Rapid Acetylcholinesterase staining.

[GUTIERREZ, JUAN]

The only replacement for isoOMPA that I know of is Ethopropazine and it's no longer available the last I heard.  Good luck.
Juan

	-----Original Message----- 
	From: Richard Hessler [mailto:RHESSLER <@t> mail.mcg.edu] 
	Sent: Fri 1/23/2004 6:12 AM 
	To: histonet <@t> lists.utsouthwestern.edu 
	Cc: 
	Subject: [Histonet] Rapid Acetylcholinesterase staining.
	
	

	I was wondering if anyone had experience with rapid acetylcholinesterase
	staining for the interoperative analysis of GI biopsies for
	Hirshprung's/Neural Intestinal Dysplasia. I found 3 rapid protocols in
	the litterature, all of which use iso-octamethylpyrophosphoramide
	(isoOMPA). According to Sigma this chemical is used by the military in
	nerve gas production and if fatal on contact with skin or inhalation;
	not something we can use in the frozen section room!
	Any less toxic alternatives out there?
	
	Richard B Hessler, MD
	Chief, Section of Anatomic Pathology
	Associate Professor of Pathology and Neurology
	The Medical College of Georgia