[Histonet] aldehyde fuchsin

[Geoff McAuliffe]

    It's beautiful. Fix with Bouin's, wash out the picric acid with 
several changes of 50% ethanol. The stain is easy to make and apply. 
Oxidation of the sections is not needed if your basic fuchsin has enough 
pararosanaline in it. Pararosanaline is the molecule that complexes with 
the aldehyde to form the stain. In the past, some lots of basic fuchsin 
did not contain sufficient pararosanaline to give a good reaction so be 
sure your dye has a high percentage of pararosanaline. Or just order a 
bottle of Pararosanaline, be sure it is Certified by the Biological 
Stain Commission.
    Paraldehyde is a controlled substance and can be difficult to 
obtain, if you are at a hospital the pharmacy may have it in little 
glass ampoules which is good because in needs to be fresh. Acetaldehyde, 
which is easier to get, can be substituted for paraldehyde but you need 
to use three times as much.
See Buehner, Nettleton and Longley, J. Histochem. Cytochem 27:782-787, 
1979 for details.

Geoff

Rose Lederer wrote:

>Dear experts, 
>
>How good is the (Gomory's) Aldehyde Fuchsin stain to demonstrate the
>islet cells? 
>Is it used at all?
>
>Sorry to bother you but I just wonder if it's worth doing
>
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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