[Histonet] E1Ajb0N-0004YT-Qs@mk-webmail-2.b2b.uk.tiscali.com Micronuclei

[Stanley.Lupo <@t> gsk.com]

Stanley.Lupo <@t> GSK.Com
Safety Assessment
Mail Code:  UE0461
Phone:  610 270-7340
Fax:  610 270-7202

It's been years since I've done the Micronucleus Test in rodents, but we 
always had good differentiation of polychromatic and normochromatic 
erythrocytes with the traditional May Gruenwald and Giemsa staining as 
recommended by the early investigators, Boller and Schmid, Heddle, etc. 
NCE's were pink to orange, while PCE's were blue.  Micronuclei appeared 
dark purple and were unmistakeable.




"tony filips" <otis2k <@t> yahoo.com>

Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
22-Jan-2004 13:26

 
 

        To:     histonet

        cc: 
        Subject:        [Histonet] E1Ajb0N-0004YT-Qs <@t> mk-webmail-2.b2b.uk.tiscali.com Micronuclei

A few years ago when I was doing Gene Tox work we used
Acridine orange (pre-coated slides) on blood smears
(then coverslipped) for NCE's PCE's and Micronuclei.

caveat- the slide folders had to be stored in the
freezer (in ziploc bags) as the stain fades with
prolonged time at R.T.

I'll post the procedure if I can find it.

Anthony Filipunas  (otis2k <@t> yahoo.com)

Histology Manager
MPI Research
Mattawan, MI



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