[Histonet] Rapid Acetylcholinesterase staining.

[Richard Hessler]

I was wondering if anyone had experience with rapid acetylcholinesterase
staining for the interoperative analysis of GI biopsies for
Hirshprung's/Neural Intestinal Dysplasia. I found 3 rapid protocols in
the litterature, all of which use iso-octamethylpyrophosphoramide
(isoOMPA). According to Sigma this chemical is used by the military in
nerve gas production and if fatal on contact with skin or inhalation;
not something we can use in the frozen section room!
Any less toxic alternatives out there?

Richard B Hessler, MD
Chief, Section of Anatomic Pathology
Associate Professor of Pathology and Neurology
The Medical College of Georgia
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