[Histonet] CD13 -- FFPE immunohistochemistry

[Bryan Hewlett]

Alex,

I haven't tried it, but Novocastra list the following;

NCL-CD13-304, clone 38C12, which is reported to work on FFPE following HIER
in citrate buffer pH6.0

Worth a try!

Regards,

Bryan

----- Original Message -----
From: "Alex Knisely" <alex.knisely <@t> kcl.ac.uk>
To: "HistoNet Server" <histonet <@t> pathology.swmed.edu>
Sent: Wednesday, January 21, 2004 6:31 AM
Subject: [Histonet] CD13 -- FFPE immunohistochemistry


> CD13, also called "aminopeptidase N", is a white-cell antigen usually
> assessed by flow cytometry.
>
> I'd like to look at it in FFPE materials.
>
> A search through ABCAM has not found a reference to a commercially
> available source of an anti-CD13 antibody that works in FFPE sections.
>
> Can anyone recommend a source and protocol for such an antibody?
>
> Best thanks in advance
>
> Alex K
>
>
> Alex Knisely, MD
> Consultant Histopathologist
>
> alex.knisely <@t> kcl.ac.uk
>
> Institute of Liver Studies
> King's College Hospital
> Denmark Hill
> London  SE5 9RS  UK
>
> +44 (0)20 - 7346 - 3125 telefax
> +44 (0)20 - 7346 - 4627 office
>
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