[Histonet] GMS Staining - P.S.

[Stapf, Ross]

You may have a bad batch of charged slides.  This is too common.  In my
opinion you should not be able to float a section back off of a charged
slide.  Unfortunately far too often you can.  I'm sure you have checked
your oven temperature. 

At my former lab we had trouble with immuno tissue coming off the
slides.  We tried everything including extending the time in the oven,
and changing brands and types of adhesive slides.  Finally someone
suggested leaving the slides to air dry upright for a couple of hours
before putting them in the oven.  The theory was that if water was under
the tissue and the slides were put in the oven the water wouldn't really
escape because the edges of the paraffin section would melt first
trapping the water and causing the tissue to be more likely to lift off
of the slide.  I don't know how true that is, but we tried it and it
worked.  We left our immuno's to sit for 2 hours before putting them in
the oven (forced air) at 60 C for 30 minutes.  Most tissue that wasn't
badly processed would then survive even the more extreme unmasking
solutions.

It is worth a try if you can't find another reason for your problem.

Ross M Stapf
Histopathology Manager
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246
214-820-2465 
214-820-4110 fax
RossS <@t> baylorhealth.edu



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Dndsomi <@t> aol.com
Sent: Wednesday, January 21, 2004 2:37 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] GMS Staining - P.S.


Sorry, I should have mentioned that we are using charged slides.
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