[Histonet] Embedding W/WO Melted Paraffin
DMBCMP <@t> aol.com
DMBCMP <@t> aol.com
Tue Jan 20 21:37:12 CST 2004
Hi, everyone:
In order to settle a difference of opinion between generations of trained
Histotechs in my lab, may I have feedback from anyone interested in responding?
Do you cover the blocks in melted paraffin in the embedding centre reservior
and hold them that way while embedding? or....
Do you dump them in the warm reservior "dry" (not covered in melted paraffin)
and embed them that way?
Do you consider this "dry" method as bad technique since a tiny biopsy
specimen MAY not be noticed as the paraffin quickly solidifies?
We have a dispute. I have researched every book I can find and there is no
reference to it anywhere. A newly trained tech that came to work for us said
no mention was made during her training period. Some techs did...and some
didn't. As a tech of nearly 27 years, I find this practice to be just asking for
trouble. I was trained to keep everything melted. There seems to be some
argument against keeping the cassettes in the melted paraffin, claiming it "cooks"
the biopsies. I don't buy it, but what are the opinions of others?
It seems so basic to me. I hope this does not come across as frivilous.
Thanks for your input.
Dannie Blake HT(ASCP) Histology Lead Tech
Fresno Community Medical Centre
Fresno, California
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