[Histonet] C4d antibody

[Stapf, Ross]

I made the same mistake for the first month I was here.  

You should have seen the look on my immuno techs' faces when after they
asked me to find a new source for C4D, I handed them a whole bunch of
CD4 spec sheets.

Ross M Stapf
Histopathology Manager
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246
214-820-2465 
214-820-4110 fax
RossS <@t> baylorhealth.edu



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nick
Kirk
Sent: Tuesday, January 20, 2004 1:43 PM
To: Histonet
Subject: RE: [Histonet] C4d antibody


Oops, must need my eyes testing, just read this again and noticed it is
an enquiry for C4d not CD4. Ignore the previous mumblings of an addled
person.

Nick Kirk

-----Original Message-----
From: Nick Kirk [mailto:nick.kirk3 <@t> btopenworld.com]
Sent: 20 January 2004 19:41
To: Histonet
Subject: RE: [Histonet] C4d antibody


We use Novocastra's CD4 on FFPE sections using Vector's Antigen
Unmasking Fluid in a pressure cooker and Dako's ChemMate HRP detection
kit which uses DAB. We use our routine method and have few problems
getting it to work. The biggest problem is the availability of good
quality tonsil control material, that hasn't been fixing for years in
the cupboard.

Nick Kirk
Histopathology
Hinchingbrooke Hospital
Huntingdon
England

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
Featherstone, Annette
Sent: 20 January 2004 18:41
To: 'Instrumedics'; HistoNet Server
Subject: [Histonet] C4d antibody


Hi

Anyone out there using C4d antibody? Have to work it up and want to know
if there are any tips to make this work as they haven't been able to get
it to work right. Manufacturer recommends Alk Phos, but I was hoping to
get it to work with DAB. Any suggestions? Thanks Annette Featherstone
HT/MLT




-----Original Message-----
From: Instrumedics [mailto:info <@t> instrumedics.com]
Sent: Tuesday, January 20, 2004 12:44
To: HistoNet Server
Subject: [Histonet] tissue carryover


One of the advantages of the Cryo-Vac-Away vacuum system for the
cryostat is that the debris generated during the trimming step is
suctioned away as  it is generated at the blockface. This substantially
reduces the chances that any sections will cling to the underside of the
knife which can then can carryover to the following case.

Bernice
schiller <@t> instrumedics.com



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