[Histonet] C4d antibody

[Nick Kirk]

We use Novocastra's CD4 on FFPE sections using Vector's Antigen Unmasking
Fluid in a pressure cooker and Dako's ChemMate HRP detection kit which uses
DAB.
We use our routine method and have few problems getting it to work.
The biggest problem is the availability of good quality tonsil control
material, that hasn't been fixing for years in the cupboard.

Nick Kirk
Histopathology
Hinchingbrooke Hospital
Huntingdon
England

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
Featherstone, Annette
Sent: 20 January 2004 18:41
To: 'Instrumedics'; HistoNet Server
Subject: [Histonet] C4d antibody


Hi

Anyone out there using C4d antibody? Have to work it up and want to know if
there are any tips to make this work as they haven't been able to get it to
work right. Manufacturer recommends Alk Phos, but I was hoping to get it to
work with DAB. Any suggestions? Thanks
Annette Featherstone HT/MLT




-----Original Message-----
From: Instrumedics [mailto:info <@t> instrumedics.com]
Sent: Tuesday, January 20, 2004 12:44
To: HistoNet Server
Subject: [Histonet] tissue carryover


One of the advantages of the Cryo-Vac-Away vacuum system for the cryostat is
that the debris generated during the trimming step is suctioned away as  it
is generated at the blockface. This substantially reduces the chances that
any sections will cling to the underside of the knife which can then can
carryover to the following case.

Bernice
schiller <@t> instrumedics.com



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

CONFIDENTIALITY NOTICE:
This email transmission and any documents, files,
or previous e-mail messages attached to it are
confidential and intended solely for the use of the
individual or entity to whom they are addressed.
If you are not the intended recipient, or a person
responsible for delivering it to the intended recipient,
you are hereby notified that any further review,
disclosure, copying, dissemination, distribution, or
use of any of the information contained in or attached
to this e-mail transmission is strictly prohibited.
If you have received this message in error, please
notify the sender immediately by e-mail, discard
any paper copies, and delete all electronic files
of the message. If you are unable to contact the
sender or you are not sure as to whether you
are the intended recipient, please e-mail
ISTSEC <@t> KaleidaHealth.org or call (716) 859-7777.



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet