[Histonet] carryover on slides

Marshall Terry Dr, Consultant Histopathologist Terry.Marshall <@t> rothgen.nhs.uk
Tue Jan 20 09:00:59 CST 2004

Surely all are examples of carryover.
Carryover is anything that "didn't ought to be there" and has come from another specimen.
It must be difficult to monitor, and the most difficult (i.e. impossible) is the like-to-like tissue carryover, that is to say, a bit of A's endometrium appearing on B's slide.
Cut-up and water bath are the most likely origins, and can be minimised by scrupulous cleanliness.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
        terry.marshall <@t> rothgen.nhs.uk

-----Original Message-----
From: Gayle Callis [mailto:gcallis <@t> montana.edu]
Sent: 20 January 2004 14:45
To: Vickroy, Jim; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] carryover on slides

Could you define carryover?  Do you mean cells, tissue fragments, stains
from one staining dish to another?  

At 07:40 AM 1/20/2004 -0600, you wrote:
>Has anyone ever seen any articles on what is an acceptable rate of carryover
>on routine slides?  We have been monitoring our carryover rate and our
>pathologists wondered if anyone had ever seen a study on carryover rates.
>James R. Vickroy BS, HT (ASCP)
>Technical Supervisor, Surgical Pathology
>vickroy.jim <@t> mhsil.com
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
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