[Histonet] Re: Histonet Digest, Vol 2, Issue 19
peptolab
peptolab <@t> hamptons.com
Fri Jan 16 12:10:24 CST 2004
This weather sucks. I slid sideways into a telephone pole at 25 MPH and bent
my right front wheel inwards trying to get to the lab at 4:45 AM yesterday.
Five day weekend for me.
Jeff Silverman
----- Original Message -----
From: <histonet-request <@t> lists.utsouthwestern.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, January 16, 2004 1:00 PM
Subject: Histonet Digest, Vol 2, Issue 19
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> Today's Topics:
>
> 1. Re: glycol methacrylate and sectioning (Gayle Callis)
> 2. Hope you all aren't freezing in the east!
> (Morken, Tim - Labvision)
> 3. RE: Hope you all aren't freezing in the east! (Weems, Joyce)
> 4. RE: Friday Humor (Weems, Joyce)
> 5. Ht Certification Route 3 (mprice26 <@t> juno.com)
> 6. RE: Formalin down the sink (BRobert <@t> ameripath.com)
> 7. Staining nerve fibres in human palate (anclg)
> 8. RE: Hope you all aren't freezing in the east!
> (Jackie.O'Connor <@t> abbott.com)
> 9. Re: Ht Certification Route 3 (Vinnie Della Speranza)
> 10. (no subject) (Orlaith Brennan)
> 11. RE: Hope you all aren't freezing in the east!
> (tbergeron <@t> criver.com)
> 12. Please unsubscribe (Martha Ward)
> 13. Detection level (Bertrand Lefort)
> 14. RE: Hope you all aren't freezing in the east! (Linke_Noelle)
> 15. RE: Hope you all aren't freezing in the east! (Bartlett, Jeanine)
> 16. Re: (no subject) (Gayle Callis)
> 17. RE: Hope you all aren't freezing in the east!
> (Dunikoski, Leonard PhD)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 16 Jan 2004 08:59:57 -0700
> From: Gayle Callis <gcallis <@t> montana.edu>
> Subject: Re: [Histonet] glycol methacrylate and sectioning
> To: Geoff McAuliffe <mcauliff <@t> umdnj.edu>,
> Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <3.0.6.32.20040116085957.00bd9f50 <@t> gemini.msu.montana.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Having done glycol methacrylate, I agree with Geoff's comments. I have a
> feeling 30 um sections of GMA embedded tissues would shatter coming off
the
> knife, and you would probably need an expensive tungsten carbide knife to
> even attempt this and a really powerful sliding microtome. We always
> reserved GMA methods for 3 um or less thickness, thicker we went back to
> paraffin, OR with bone, ground and polished sections in
methylmethacrylate,
> but this would NOT be suitable for brain work, rather mineralized bone
> sections.
>
> Good luck.
>
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
>
>
>
> At 10:16 AM 1/16/2004 -0500, you wrote:
> >Hi David:
> >
> > If you want 30 micron sections of brain why not frozen sections? or
> >celloidin sections? I have never heard of cutting glycol methacrylate
> >that thick, I always thought the purpose of plastic sections was thin
> >sections. Perhaps the "plastic" people on this list can provide advice.
> >
> >Geoff
> >
> >David Laidley wrote:
> >
> >>This may be a stupid question but can a sliding microtome cut brain
> tissue at 30 um when embedded in glycol methacrylate. I know that there
> exists rotory microtomes that cut glycol methacrylate but I can't seem to
> find any information about sliding microtomes.
> >>
> >>If you can cut 30 um sections of brain tissue in glycol methacrylate
then
> does anyone have any tips on how this might be done (well). Or at least
any
> tips or tricks you may have picked up over the years that may make the
> process go more smoothly.
> >>
> >>David Laidley (MSc student)
> >>Memorial University of Newfoundland
> >>Division of Basic Medical Sciences, Neuroscience
> >>dave_laidley <@t> yahoo.ca
> >>
> >>
> >>
> >>---------------------------------
> >>Post your free ad now! Yahoo! Canada Personals
> >>_______________________________________________
> >>Histonet mailing list
> >>Histonet <@t> lists.utsouthwestern.edu
> >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >>
> >>
> >>
> >
> >--
> >--
> >**********************************************
> >Geoff McAuliffe, Ph.D.
> >Neuroscience and Cell Biology
> >Robert Wood Johnson Medical School
> >675 Hoes Lane, Piscataway, NJ 08854
> >voice: (732)-235-4583; fax: -4029
> >mcauliff <@t> umdnj.edu
> >**********************************************
> >
> >
> >
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet <@t> lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 16 Jan 2004 08:35:48 -0800
> From: "Morken, Tim - Labvision" <tpmorken <@t> labvision.com>
> Subject: [Histonet] Hope you all aren't freezing in the east!
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <0556BE8AC5551E4E8AF6BB9E42509BA203571F <@t> usca0082k08.labvision.apogent.com>
>
> Content-Type: text/plain
>
> I just want all of our collegues on the east coast (of US) to know we on
the
> west coast empathize with your freezing cold weather and hope you get
> through it OK. We are having really bad weather here too. It's cloudy
today.
>
> Tim Morken
> Pleasanton (as nice as it sounds), California
>
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 16 Jan 2004 11:39:09 -0500
> From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
> Subject: RE: [Histonet] Hope you all aren't freezing in the east!
> To: "'Morken, Tim - Labvision'" <tpmorken <@t> labvision.com>,
> Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B44019798E0 <@t> exch4.sjha.org>
> Content-Type: text/plain; charset="utf-8"
>
> But in Hotlanta we are in the sun and shirt sleeves. Hopefully it will
> not
> blow down this way!
>
> j
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Morken,
> Tim - Labvision
> Sent: Friday, January 16, 2004 11:36 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Hope you all aren't freezing in the east!
>
>
> I just want all of our collegues on the east coast (of US) to know we on
> the
> west coast empathize with your freezing cold weather and hope you get
> through it OK. We are having really bad weather here too. It's cloudy
> today.
>
> Tim Morken
> Pleasanton (as nice as it sounds), California
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
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> replying to the message and deleting it from your computer.
> Thank you. Saint Josephâ?Ts Health System, Inc.
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 16 Jan 2004 11:40:08 -0500
> From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
> Subject: RE: [Histonet] Friday Humor
> To: 'Victor Tobias' <victor <@t> pathology.washington.edu>,
> Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B44019798E1 <@t> exch4.sjha.org>
> Content-Type: text/plain; charset="utf-8"
>
> Don't you be ugly. Anyway, blondes have more fun...
>
> Have a great weekend everyone! j
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Victor
> Tobias
> Sent: Friday, January 16, 2004 10:49 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Friday Humor
>
>
> This is courtesy of my blonde wife. Last night I finally ported our cell
>
> numbers from AT&T to Verizon. After being home for awhile my wife asked
> how close were the new numbers to our old number.
>
> Victor
>
> --
> Victor Tobias
> Clinical Applications Analyst
> Dept of Pathology
> University of Washington Medical Center
> 206-543-4823
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> Confidentiality Notice ** The information contained in this message may
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> contents of this information is strictly prohibited. If you have
> received this communication in error, please notify us immediately by
> replying to the message and deleting it from your computer.
> Thank you. Saint Josephâ?Ts Health System, Inc.
>
>
> ------------------------------
>
> Message: 5
> Date: Fri, 16 Jan 2004 16:43:08 GMT
> From: mprice26 <@t> juno.com
> Subject: [Histonet] Ht Certification Route 3
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20040116.084317.5566.1031929 <@t> webmail18.nyc.untd.com>
> Content-Type: text/plain
>
>
> Histonetters,
> I know this has been discussed several times. And I did save this info but
on my home computer.
>
> The question:
> I have an employee that her 2 year OJT will not be complete until December
of this year. So, she will not be able to become an registered HT through
Route 3 Is this correct?
>
> Thank you.
>
> Marsha Price
>
> ________________________________________________________________
> The best thing to hit the internet in years - Juno SpeedBand!
> Surf the web up to FIVE TIMES FASTER!
> Only $14.95/ month - visit www.juno.com to sign up today!
>
>
>
> ------------------------------
>
> Message: 6
> Date: Fri, 16 Jan 2004 11:48:21 -0500
> From: BRobert <@t> ameripath.com
> Subject: RE: [Histonet] Formalin down the sink
> To: bamur <@t> alaska.net, Histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <DAC13D6F8712B14AAF970CF9B9E59304047CC05D <@t> rbo-mail.ameripath>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Barbara,
> You know like me how hazardous formalin is, the best, safest and most
> economical way to dispose of it based on my experience is to neutralise it
> first and then dispose of it down the drain after testing the completed
> reaction.
> We use Formalex from American Bio Safety cat# FX-05. We neutralize at the
> end of the day, let sit overnight and test and dump the following morning.
> It works really well.
> Good luck!
>
> Brigitte Robert
> Histology Lab. Manager
> A.P.M.G Inc. Los Gatos, CA
>
> -----Original Message-----
> From: Barbara Murray [mailto:bamur <@t> alaska.net]
> Sent: Thursday, January 15, 2004 5:04 PM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Formalin down the sink
>
>
> Greetings,
> We have been using a solidifier for our formalin, putting it into
biohazard
> boxes for pickup by the housekeeping dept. We were told by our Safety
> Officer that we can now pour it down the drain with lots of running water.
> For the ones of you who are using formalin, how are you disposing of it?
> Thanks for your replies. Have a great day and weekend!
>
> Barbara A. Murray,HT.(ASCP)
> The Alaska Native Medical Center
> Anchorage, Alaska
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 7
> Date: Fri, 16 Jan 2004 17:08:47 -0000
> From: anclg <chris.goodall <@t> bristol.ac.uk>
> Subject: [Histonet] Staining nerve fibres in human palate
> To: histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000701c3dc53$67c66f40$8f6ede89 <@t> hist143>
> Content-Type: text/plain; charset="us-ascii"
>
> Does anyone have any suggestions as to the most reliable way of
> staining nerve fibres in a complete human palate? I was wondering about
> sudan black on frozen sections, or one of the silver stains for paraffin
> sections.I have been asked to do this in order to reconstruct a 3D image
> of the nerves. Thanks.
> Chris Goodall
>
>
>
>
> ------------------------------
>
> Message: 8
> Date: Fri, 16 Jan 2004 10:58:31 -0600
> From: Jackie.O'Connor <@t> abbott.com
> Subject: RE: [Histonet] Hope you all aren't freezing in the east!
> To: "Weems, Joyce" <JWEEMS <@t> sjha.org>
> Cc: Histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OF1AFA3FCE.0E54B488-ON86256E1D.005CE2BE <@t> northamerica.intra.abbott.com>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Chicago is a balmy 25, with freezing rain and sleet predicted for this
> evening. Perfect. Why do we live here?
>
> Does anyone have a scientific reason for temps to get to -100F (windchill
> is what I heard for the East). Don't bugs die when it's warmer?
> Don't some just stay frozen and pop back up again in the spring? I don't
> see any reason for it to be this cold!!! (I don't see a scientific reason
> to support the existence of mosquitoes, either)
>
> Jacqueline M. O'Connor HT(ASCP)
> Abbott Laboratories
> Global Pharmaceutical Research and Development
> Discovery Chemotheraputics
> 847.938.4919
>
>
>
>
>
> "Weems, Joyce" <JWEEMS <@t> sjha.org>
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 01/16/2004 10:39 AM
>
>
> To: "'Morken, Tim - Labvision'" <tpmorken <@t> labvision.com>,
> Histonet <@t> lists.utsouthwestern.edu
> cc:
> Subject: RE: [Histonet] Hope you all aren't freezing in the
east!
>
>
> But in Hotlanta we are in the sun and shirt sleeves. Hopefully it will
> not
> blow down this way!
>
> j
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Morken,
> Tim - Labvision
> Sent: Friday, January 16, 2004 11:36 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Hope you all aren't freezing in the east!
>
>
> I just want all of our collegues on the east coast (of US) to know we on
> the
> west coast empathize with your freezing cold weather and hope you get
> through it OK. We are having really bad weather here too. It's cloudy
> today.
>
> Tim Morken
> Pleasanton (as nice as it sounds), California
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> Confidentiality Notice ** The information contained in this message may
> be privileged and is confidential information intended for the use of
> the addressee listed above. If you are neither the intended recipient
> nor the employee or agent responsible for delivering this message to the
> intended recipient, you are hereby notified that any disclosure,
> copying, distribution or the taking of any action in reliance on the
> contents of this information is strictly prohibited. If you have
> received this communication in error, please notify us immediately by
> replying to the message and deleting it from your computer.
> Thank you. Saint Joseph's Health System, Inc.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Fri, 16 Jan 2004 12:06:38 -0500
> From: "Vinnie Della Speranza" <dellav <@t> musc.edu>
> Subject: Re: [Histonet] Ht Certification Route 3
> To: <mprice26 <@t> juno.com>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <s007d3da.094 <@t> cl.musc.edu>
> Content-Type: text/plain; charset=US-ASCII
>
> Marsha,
> It would seem to me that if your employee's application to take the exam
is turned in before Jan1, she will be eligible however I would urge you to
call the BOR to confirm this information. If for example she becomes
eligible on Dec 31, I would hope that a faxed application may be acceptable
however you may learn that the BOR is allowing a grace period to prevent
individuals in this situation from being excluded. please let us know what
you learn
>
> Vinnie
>
> Vinnie Della Speranza
> Manager for Anatomic Pathology Services
> Medical University of South Carolina
> 165 Ashley Avenue Suite 309
> Charleston, SC 29425
> Ph: 843-792-6353
> fax: 843-792-8974
>
> >>> <mprice26 <@t> juno.com> 01/16/04 11:43AM >>>
>
> Histonetters,
> I know this has been discussed several times. And I did save this info but
on my home computer.
>
> The question:
> I have an employee that her 2 year OJT will not be complete until December
of this year. So, she will not be able to become an registered HT through
Route 3 Is this correct?
>
> Thank you.
>
> Marsha Price
>
> ________________________________________________________________
> The best thing to hit the internet in years - Juno SpeedBand!
> Surf the web up to FIVE TIMES FASTER!
> Only $14.95/ month - visit www.juno.com to sign up today!
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Fri, 16 Jan 2004 17:12:27 -0000
> From: "Orlaith Brennan" <OBrennan1 <@t> rcsi.ie>
> Subject: [Histonet] (no subject)
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <3E9C9E677B39D249BA3B0A0AB81ED94D08ABAB <@t> excha_stgsan1.rcsi-internal.ie>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
>
>
> > I'm working on apoptosis in ovine osteocytes. We plan on doing TUNEL
staining and also Caspase-3 antibody staining. I was wondering how long from
the point the animal dies do you have to freeze the samples before the DNA
and proteins will start to deteriorate? Is freezing necessarily the best
method of preserving the samples, or should we consider an alternative? Has
anyone performed either tunel or caspase 3 staining on undecalcified bone
sections?
> >
> > We also plan on looking at other proteins within the bone, inparticular
nitric oxide synthase. Again, how long before this protein begins to
denature? Does anyone have a protocol for the preservation of bone proteins?
> >
> > Is there a commercial storage solution for proteins (similar to ambions
RNALater)? If so would this be effective for a couple of hours to allow the
samples be transported?
> >
> > Has anyone used a large bone biopsy set?
> >
> > Any advice?
> >
> > Thank You,
> >
> > Orlaith Brennan
> >
> >
> >
> >
> --------------------------------------------------------------------------
------------------------------------------
> This email and any files transmitted with it are confidential and
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>
> ------------------------------
>
> Message: 11
> Date: Fri, 16 Jan 2004 12:12:37 -0500
> From: tbergeron <@t> criver.com
> Subject: RE: [Histonet] Hope you all aren't freezing in the east!
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OF207A502A.38B64314-ON85256E1D.005E24C8-85256E1D.005EA15E <@t> criver.com>
> Content-Type: text/plain; charset=US-ASCII
>
>
>
>
>
> Those of us here in the North East would GLADLY even take any warmer
> weather that you can send this way...
>
> Last night they said we were going to get -30 to -60 wind chills
over
> night.. This am, not sure what the wind chill was but along with it being
> -10, it wasn't too pleasant a day that's for sure..
>
>
> Tracy E. Bergeron, HT(ASCP)
> Histology Laboratory
> Charles River Laboratories
> Wilmington, MA
> 978-658-6000
> x-1229
>
>
> |---------+----------------------------------------->
> | | Jackie.O'Connor <@t> abbott.com |
> | | Sent by: |
> | | histonet-bounces <@t> lists.utsouth|
> | | western.edu |
> | | |
> | | |
> | | 01/16/2004 11:58 AM |
> | | |
> |---------+----------------------------------------->
>
>---------------------------------------------------------------------------
------------------------------------|
> |
|
> | To: "Weems, Joyce" <JWEEMS <@t> sjha.org>
|
> | cc: Histonet <@t> lists.utsouthwestern.edu
|
> | Subject: RE: [Histonet] Hope you all aren't freezing in the
east! |
>
>---------------------------------------------------------------------------
------------------------------------|
>
>
>
>
> Chicago is a balmy 25, with freezing rain and sleet predicted for this
> evening. Perfect. Why do we live here?
>
> Does anyone have a scientific reason for temps to get to -100F (windchill
> is what I heard for the East). Don't bugs die when it's warmer?
> Don't some just stay frozen and pop back up again in the spring? I don't
> see any reason for it to be this cold!!! (I don't see a scientific reason
> to support the existence of mosquitoes, either)
>
> Jacqueline M. O'Connor HT(ASCP)
> Abbott Laboratories
> Global Pharmaceutical Research and Development
> Discovery Chemotheraputics
> 847.938.4919
>
>
>
>
>
> "Weems, Joyce" <JWEEMS <@t> sjha.org>
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 01/16/2004 10:39 AM
>
>
> To: "'Morken, Tim - Labvision'" <tpmorken <@t> labvision.com>,
> Histonet <@t> lists.utsouthwestern.edu
> cc:
> Subject: RE: [Histonet] Hope you all aren't freezing in the
> east!
>
>
> But in Hotlanta we are in the sun and shirt sleeves. Hopefully it will
> not
> blow down this way!
>
> j
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Morken,
> Tim - Labvision
> Sent: Friday, January 16, 2004 11:36 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Hope you all aren't freezing in the east!
>
>
> I just want all of our collegues on the east coast (of US) to know we on
> the
> west coast empathize with your freezing cold weather and hope you get
> through it OK. We are having really bad weather here too. It's cloudy
> today.
>
> Tim Morken
> Pleasanton (as nice as it sounds), California
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> Confidentiality Notice ** The information contained in this message may
> be privileged and is confidential information intended for the use of
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> ------------------------------
>
> Message: 12
> Date: Fri, 16 Jan 2004 12:17:32 -0500
> From: "Martha Ward" <mward <@t> wfubmc.edu>
> Subject: [Histonet] Please unsubscribe
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <61135F0455D33347B5AAE209B903A30403262346 <@t> EXCHVS2.medctr.ad.wfubmc.edu>
>
> Content-Type: text/plain; charset="US-ASCII"
>
> I will be out of the office from 1/16 to 1/29.
> Martha Ward
>
>
> ------------------------------
>
> Message: 13
> Date: Fri, 16 Jan 2004 12:18:07 -0400
> From: "Bertrand Lefort" <lefortb <@t> crhsc.umontreal.ca>
> Subject: [Histonet] Detection level
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <DKEBIFOIMGKIKPHKAIHIMEIECAAA.lefortb <@t> crhsc.umontreal.ca>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello,
>
> I am used to do immuno-cyto-fluorecence on different receptors and
peptides.
>
> I would like to know if there is someone who has used different technology
> to find the detection level of this technique.
>
> I imagine that I can see a signal if there is a certain number of
molecules
> (10000 - 100000 receptor per cells ???).
>
> Have someone an idea of this number ?
>
> Bertrand
>
>
>
>
> ------------------------------
>
> Message: 14
> Date: Fri, 16 Jan 2004 09:23:56 -0800
> From: "Linke_Noelle" <Linke_Noelle <@t> Allergan.com>
> Subject: RE: [Histonet] Hope you all aren't freezing in the east!
> To: "Morken, Tim - Labvision" <tpmorken <@t> labvision.com>,
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <D475C767BF2F6D438FD30E3984FCBB110C159D <@t> irmail121.irvine.allergan.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Tim is right. It's kind of cloudy here in southern California too, and
> it's only supposed to get up to 70 today. Very sad.
>
> Noelle (damn glad she left Boston) Linke, BS, HTL(ASCP)
> Allergan, Inc
> 2525 Dupont Drive RD-2A
> Irvine, CA 92612
> 714-246-5568
>
>
> -----Original Message-----
> From: Morken, Tim - Labvision [mailto:tpmorken <@t> labvision.com]
> Sent: Friday, January 16, 2004 8:36 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Hope you all aren't freezing in the east!
>
> I just want all of our collegues on the east coast (of US) to know we on
> the
> west coast empathize with your freezing cold weather and hope you get
> through it OK. We are having really bad weather here too. It's cloudy
> today.
>
> Tim Morken
> Pleasanton (as nice as it sounds), California
>
> _______________________________________________
> Histonet mailing list
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 15
> Date: Fri, 16 Jan 2004 12:30:57 -0500
> From: "Bartlett, Jeanine" <jqb7 <@t> cdc.gov>
> Subject: RE: [Histonet] Hope you all aren't freezing in the east!
> To: "Morken, Tim - Labvision" <tpmorken <@t> labvision.com>,
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <CB857F6460D42E4AAEA195054A25406C08112E <@t> m-ncid-2.ncid.cdc.gov>
> Content-Type: text/plain; charset="UTF-8"
>
> Hmmmm....Here in Atlanta it's chilly in the mornings (around 32) and
lovely in the afternoon (around 55). Not as warm as California but fewer
fires, earthquakes, floods and mudslides!
>
> Jeanine Bartlett
> CDC/Atlanta
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Morken, Tim -
Labvision
> Sent: Fri 1/16/2004 11:35 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Cc:
> Subject: [Histonet] Hope you all aren't freezing in the east!
>
>
>
> I just want all of our collegues on the east coast (of US) to know we on
the
> west coast empathize with your freezing cold weather and hope you get
> through it OK. We are having really bad weather here too. It's cloudy
today.
>
> Tim Morken
> Pleasanton (as nice as it sounds), California
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 16
> Date: Fri, 16 Jan 2004 10:44:51 -0700
> From: Gayle Callis <gcallis <@t> montana.edu>
> Subject: Re: [Histonet] (no subject)
> To: "Orlaith Brennan" <OBrennan1 <@t> rcsi.ie>,
> Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <3.0.6.32.20040116104451.00bcfbc8 <@t> gemini.msu.montana.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> I suggest you access RA Dodds publications on doing undecalcified bone
> frozen sections.
>
> By the way, what is a "large bone biopsy set"? A trephine?
>
> At 05:12 PM 1/16/2004 -0000, you wrote:
> >
> >
> >
> >> I'm working on apoptosis in ovine osteocytes. We plan on doing TUNEL
> staining and also Caspase-3 antibody staining. I was wondering how long
> from the point the animal dies do you have to freeze the samples before
the
> DNA and proteins will start to deteriorate? Is freezing necessarily the
> best method of preserving the samples, or should we consider an
> alternative? Has anyone performed either tunel or caspase 3 staining on
> undecalcified bone sections?
> >>
> >> We also plan on looking at other proteins within the bone, inparticular
> nitric oxide synthase. Again, how long before this protein begins to
> denature? Does anyone have a protocol for the preservation of bone
proteins?
> >>
> >> Is there a commercial storage solution for proteins (similar to ambions
> RNALater)? If so would this be effective for a couple of hours to allow
the
> samples be transported?
> >>
> >> Has anyone used a large bone biopsy set?
> >>
> >> Any advice?
> >>
> >> Thank You,
> >>
> >> Orlaith Brennan
> >>
> >>
> >>
> >>
>
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> >
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
>
>
>
>
>
> ------------------------------
>
> Message: 17
> Date: Fri, 16 Jan 2004 12:52:09 -0500
> From: "Dunikoski, Leonard PhD" <ldunikoski <@t> rbmc.org>
> Subject: RE: [Histonet] Hope you all aren't freezing in the east!
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <7A2DF880A7A1D511A63300508BF7D5550271AD00 <@t> es1.rbmc.org>
> Content-Type: text/plain
>
> Just remember the words of Scripture:
>
> Many are cold, but few are frozen
>
>
> Leonard K. Dunikoski, Ph.D.
> Director of Operations
> Raritan Bay Medical Center
> Perth Amboy, NJ 08861
>
>
> -----Original Message-----
> From: Morken, Tim - Labvision [mailto:tpmorken <@t> labvision.com]
> Sent: Friday, January 16, 2004 11:36 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Hope you all aren't freezing in the east!
>
> I just want all of our collegues on the east coast (of US) to know we on
the
> west coast empathize with your freezing cold weather and hope you get
> through it OK. We are having really bad weather here too. It's cloudy
today.
>
> Tim Morken
> Pleasanton (as nice as it sounds), California
>
> _______________________________________________
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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> End of Histonet Digest, Vol 2, Issue 19
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