[Histonet] PAS with and without Diastase for glycogen

[Celebre Julia]

Here's one idea..... 
Have you thought of trying a composite block made up of liver, cervix and
even kidney? 
That way all bases are covered...

> ----------
> From: 	Barnhart, Tammy[SMTP:Tbarnhart <@t> primecare.org]
> Sent: 	Friday, January 16, 2004 9:52 AM
> To: 	'FreidaC <@t> aol.com'; histonet <@t> lists.utsouthwestern.edu
> Subject: 	RE: [Histonet] PAS with and without Diastase for glycogen
> 
> Thank you all for your ideas, let me elaborate...
>  
> Our pathologists won't except cervix for a control, they want a liver
> control. When we use cervix, the stain seems to work great.  I have not
> tried skin or kidney, but maybe I can get them to except them as controls.
> We have tried spitting, it works best.  I don't feel it is a problem with
> our procedure, because it seems to work well on cervix and the Schiff's
> reaction seems to be very strong.  Freida, you may have hit the nail on
> the
> head with your comment on glycogen storage in the liver.  Autopsy tissue
> is
> about all the liver we can get.  Could be that we will never get good,
> consistent results with liver controls.  However, in my previous lab we
> did
> get consistent staining with liver.  That is what has me so perplexed.  I
> think I will try the kidney.  If alot of you respond that liver will never
> make a good control, maybe I can use that as proof to the pathologists
> that
> we need to use the cervix control.  Any other ideas....please let me know.
>  
>  
> [Barnhart, Tammy]  -----Original Message-----
> From: FreidaC <@t> aol.com [mailto:FreidaC <@t> aol.com]
> Sent: Friday, January 16, 2004 8:21 AM
> To: Tbarnhart <@t> primecare.org; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] PAS with and without Diastase for glycogen
> 
> 
> 
> Tammy:
>  
> Many livers do not contain a lot of glycogen - especially autopsy livers,
> because few are well nourished and have a lot of stored glycogen at the
> time
> of death.  A better control in my opinion is a section of cervix
> containing
> both endo- and ecto- cervix.  The stratified squamous epithelium of the
> ectocervix contains glycogen which will disappear with diastase digestion,
> while the mucous glands in the endocervix do not contain glycogen, but
> rather mucin that will be positive with the PAS.  This will not disappear
> with diastase digestion.  This is also easier control tissue to find in
> most
> institutions.
>  
> Freida Carson
> 
> 
> 
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