[Histonet] PAS with and without Diastase for glycogen
FreidaC <@t> aol.com
FreidaC <@t> aol.com
Fri Jan 16 08:20:40 CST 2004
Tammy:
Many livers do not contain a lot of glycogen - especially autopsy livers,
because few are well nourished and have a lot of stored glycogen at the time of
death. A better control in my opinion is a section of cervix containing both
endo- and ecto- cervix. The stratified squamous epithelium of the ectocervix
contains glycogen which will disappear with diastase digestion, while the
mucous glands in the endocervix do not contain glycogen, but rather mucin that will
be positive with the PAS. This will not disappear with diastase digestion.
This is also easier control tissue to find in most institutions.
Freida Carson
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