[Histonet] PGP 9.5

[Mitchell (Jean A.)]

I have been using a polyclonal PGP 9.5 for immunolabeling epidermal
neurites on 50um frozen sections of skin. Are you staining sections on
slides or free floating your sections in well plates?  I have attempted
both and by far the most consistant results are obtained by free
floating the sections in well culture plates for the entire staining
procedure.

Good luck & contact me directly if I can assist you more with this
technique.

Jean Mitchell, BS, HT (ASCP)
University of Wisconsin Hospital & Clinics
Department of Neurology
Madison, WI


-----Original Message-----
From: Chow Wai Lyn, Adeline [mailto:mdcchowa <@t> nus.edu.sg] 
Sent: Tuesday, January 13, 2004 9:53 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] PGP 9.5


Im using PGP 9.5 to stain for nerve fibres in the epidermis of skin
taken from the hypothenar region. The results have been inconsistent so
far , sometimes staining well, sometimes not. Sections are 30um and
stained overnight room temp. using DAKO envision kit. Any advice ?
Thanks..

Lyn
Research Assistant
National University of Singapore


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet