[Histonet] Re: articular cartridge Staining

[Gayle Callis]

You did not say how the articular cartilage is prepared? Frozen sections,
paraffin, decalcified, plastic sections.  Toluidine Blue or saffranin
O/Fast Green work well with paraffin. Toluidine blue is excellent with
plastic sections. IF you have decalcified with EDTA, proteoglycans are
extracted (NOT destroyed) by EDTA and tinctorial quality of these stains
changes.  You will need to control staining using an undecalcified piece of
articular cartilage so you know what might be missing. 


At 10:52 AM 1/14/2004 -0500, you wrote:
>Hi, I am currently looking for a special stain that will stain articular
>cartridge.  Does anyone have any suggestions?
>
>Jennifer A. Matalik
>Histology Lab manager
>100 technology drive
>room 271
>Pittsburgh, PA 15219
>Phone# 412-235-5215
>Fax# 412-235-5110
>matalikja <@t> upmc.edu
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)