[Histonet] stain for osteoblasts

[John Baker]

Does anyone have a protocol for something like this?

Specimen cells are fixed in 10% aqueous formaldehyde, serially 
dehydrated, the specimens are embedded in paraffin and cut into 5um 
thick cross sections for H&E or von Kossa silver nitrate/alizarin red 
staining.

The question is if anyone has a more detailed protocol for staining
for bone formation with osteoblasts fixed inside a polymer (alginate in
this case).

The protocol is wanted by a graduate student in our lab and I am 
unfamiliar with working with cells.
Thank you for any assistance with this! John

-- 


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Today is the Tomorrow you worried about Yesterday. Was
it worth it?

Birthdays are good for you. Statistics show that the
people who have the most live the longest.

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John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
400 North Ingalls, G160
Ann Arbor, MI 48109-0486
Main lab office phone:734-763-9674
Histology office:734-936-1635