[Histonet] Osteoclast stain

[Patsy Ruegg]

Paul,
If you have not fixed with formalin you could do Acid Phosphatase (TRAP) for
osteoclasts, this is the enzyme histochemical method.  I can provide you the
method if you like.  I used a kit from Sigma, I hope someone is still
selling it.  There is also an antibody but I do not have experience with it.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Paul
Keogh
Sent: Tuesday, January 13, 2004 5:33 AM
To: 'Histonet (E-mail)
Subject: [Histonet] Osteoclast stain




I have sections of   MMA embedded rat femur . I want to stain for
osteoclasts.

Can anyone recommend a tried and tested protocol please.?

Paul


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