[Histonet] ice crystal artifact

[Instrumedics]

Jackie,
Please send the pictures to us.

We developed the Gentle Jane Snap Freezer to minimize ice-crystal artifact.
The method provides not only for low temperature(LN2 @ -196C) but for
thermal exchange.

 A chrome-plated brass heat extractor is chilled in liquid nitrogen and then
placed on the Gentle Jane device. It falls at a controlled rate. For the
best freezing it contacts
the tissue first,  which is located on the top of a mound of the viscous
CryoGel  embedding medium . The tissue and the embedding medium are
frozen simultaneously.

See the details of the method on our web site  www.instrumedics.com
Please contact us with any questions

Bernice
schiller <@t> instrumedics.com


----- Original Message ----- 
From: <Jackie.O'Connor <@t> abbott.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, January 12, 2004 1:07 PM
Subject: [Histonet] Frozen section ice artifact


> Yo Histonetters, and Happy New Year.
>
> I just cut some frozen sections of solid tumors in OCT which were supposed
> to be snap frozen in liquid nitrogen after being put in plastic cryomolds.
> I have never seen such horrible morphology in my career.  I think this is
> an example of the absolute worst ice crystal artifact I have ever seen,
> but since I have never seen anything this bad before, I don't know what
> I'm seeing, you see?    I hope I've intrigued someone enough to email me
> directly, so I can send you a gif file of this atrocity.  I think the
> tissues were mishandled prior to freezing (someone else froze them) and
> I'm trying to figure out what happened so I can correct the problem.  I'd
> appreciate anyone who would like to take a look at this artifact - it's
> awful, honest!
>
> Jackie O'Connor
>

----- Original Message ----- 
From: <histonet-request <@t> lists.utsouthwestern.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, January 13, 2004 6:05 AM
Subject: Histonet Digest, Vol 2, Issue 12


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> Today's Topics:
>
>    1. Frozen section ice artifact (Jackie.O'Connor <@t> abbott.com)
>    2. particle counting (Cathy Mayton)
>    3. RE: particle counting (Barry R Rittman)
>    4. RE: particle counting (Mass Histology Service)
>    5. S San Francisco job posting (Carrie Kyle-Byrne)
>    6. RE: Frozen section ice artifact (Patsy Ruegg)
>    7. Sakura VIP 5 (Kathleen Roberts)
>    8. RE: Frozen section ice artifact (LuAnn Anderson)
>    9. RE: Frozen section ice artifact (Charles  Scouten)
>   10. RE: Frozen section ice artifact (Jackie.O'Connor <@t> abbott.com)
>   11. calculators and the HT exam (JCarpenter764 <@t> aol.com)
>   12. CPT coding for IHC (Dawson, Glen)
>   13. Parathyroid Hormone Antibody (Christina Sawrenko)
>   14. ht exam and formulas (JCarpenter764 <@t> aol.com)
>   15. RE: CPT coding for IHC (Weems, Joyce)
>   16. Re: CPT coding for IHC (Richard Cartun)
>   17. Re: Parathyroid Hormone Antibody (Richard Cartun)
>   18. RE: CPT coding for IHC (Horn, Hazel V)
>   19. RE: CPT coding for IHC (LaFriniere, Mike)
>   20. Re: ht exam and formulas (Lee & Peggy Wenk)
>   21. Re: calculators and the HT exam (Lee & Peggy Wenk)
>   22. Re: Next HT (ASCP) test (Lee & Peggy Wenk)
>   23. Re: Next HT (ASCP) test (Lee & Peggy Wenk)
>   24. Practical Exams (Lee & Peggy Wenk)
>   25. Re: Unable to get to the testing info (Lee & Peggy Wenk)
>   26. Re: Sakura VIP 5 (Joe Nocito)
>   27. Sakura VIP (DMBCMP <@t> aol.com)
>   28. RE: Sakura VIP 5 (Deltour, Douglas D.(HM2))
>   29. unsubcribe (Peter Smale)
>   30. RE: Sakura VIP 5 (Bartlett, Jeanine)
>


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