[Histonet] eye-processing

[Hans Ooms]

Dear fellow histofreaks !

For we see eye's rarely in daily routine we normally send these specimens to specialised labs. This has to be STOPPED !
So could anyone help me with the next questions please ?

What's the best way of fixing a whole eye for routine sectioning and keeping morphology and cutting capabilities of high standards ? Does anyone have any experience with fixative-injection in the enucleated eye ? (no, not in vivo.......thanks Heaven).
My experience is that when a tumor, growing from the retina towards the corpus vitreum and both are attached, the latter tends to fall off during staining and take tumorparticals with it. 

Thanks again.

Hans Ooms
The Netherlands