[Histonet] brainfix and Bielschowski

[Hans Ooms]

Hello there dear histolovers !

1.
Does anyone know what the best method is for fixation of whole brain and how long does it last generally?
Perfusion is not an option I presume.

2.
Are there colleagues who have experience with Bielschowski's silver impregnation for brainslides? There could be prenty of modifications, but what gives a good result?

Thanks in advance !

J. Ooms HT,
former labtech Rijnstate Arnhem,
sectionhead histology Pamm Eindhoven

The Netherlands