[Histonet] Steiners problems-milky staining on pt tissue

[Weems, Joyce]

Have you had any B5 fixative anywhere - tissue processed in the same
processor?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Stoll,
Kathy
Sent: Thursday, January 08, 2004 2:58 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Steiners problems-milky staining on pt tissue


Our lab has been having problems with our Steiners stain for the past
several months.  Our control slide usually turns out good, but the patient
slide has milky grey coloring under the scope.  Grossly the patient tissue
has a gold coating.  Occasionally both the patient and control slides have
background staining, but not always. 
We use the following 10% NBF, control slides cut 2-3 months ago.
We have tried the following...different batch of slides, making new
solutions (we mix our own), acid cleaning the patient slide, glass coplin
jars instead of plastic, different temperatures primarily for reducing
solution, bottled water instead of tap distilled.
This problem is recent and one that we had never had before.  
Thank you in advance for your help in solving our mystery.

Kathy Stoll, HT
St. Joseph's Community Hospital
West Bend WI


**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you have received this email in error please notify
the system manager.

This footnote also confirms that this email message has been swept by
MIMEsweeper for the presence of computer viruses.

www.mimesweeper.com
**********************************************************************


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer.
Thank you. Saint Josephs Health System, Inc.