[Histonet] RNA DNA (im)possibilities

[David Walker]

I have used both DAPI and Hoechst stains to stain DNA in FFPE tissues.
Hoechst is fairly specific to DNA (it binds in the minor groove of double
stranded DNA), although it also can bind double stranded sections of RNA (in
ribosomes and transfer RNA).  I am under the (mis?)conception that DAPI
works in much the same way.  Both of these are fluorescent dyes and they
require UV light for excitation, and they both emit in the blue region of
the spectrum.  Propidium iodide is also a fluorescent dye that works to
stain DNA and RNA.  This dye excites at 536nm (green) and emits at 617nm
(red).  The Molecular Probes web site has information on these and other
nucleic acid stains.  Here is their web address:
http://www.probes.com/handbook/sections/0801.html

David Walker

On 1/6/04 1:41, "Hans Ooms" <j.p.g.ooms <@t> zonnet.nl> wrote:

> Dear Histonetters,
> 
> First of all I Wish you all a prosperous and very healthy New Year!!!!!
> 
> Secondly, the following question keeps me busy:
> 
> what RELIABLE technique can be used to show RNA and DNA in paraffinsections ?
> 
> Thanks in advance !
> 
> Hans Ooms
> HT (The Netherlands)
> 
> 
> 
> 
> 
> 
> 
> 
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