[Histonet] Epon resin

[Mitchell (Jean A.)]

I will have to disagree with using BDMA in place of DMP 30. I find that
the final result using BDMA is chippy & brittle resin. NMA, DDSA, Epon
with DMP 30 has given me consistant results for EM with a wide variety
of tissue.

DMP 30 can be purchased in smaller bottles of 25 ml if you are concerned
with shelf life. 

Jean Mitchell
University of Wisconsin Hospital & Clinics
Department of Neurology, Neuromuscular Laboratory
Madison, WI

-----Original Message-----
From: Geoff McAuliffe [mailto:mcauliff <@t> umdnj.edu] 
Sent: Monday, January 05, 2004 10:46 AM
To: Myri37 <@t> aol.com
Cc: histonet <@t> pathology.swmed.edu
Subject: Re: [Histonet] Epon resin


Hi Myri:

    I don't know if one is better than the other and I suspect that in 
most uses these mixtures would be indistinguishable. I do concur with 
Steve's suggestion of using BDMA instead of DMP-30. The former has a 
longer shelf life.

Geoff

Myri37 <@t> aol.com wrote:

>Dear histonetters
>I wish you a healthy and happy new year 2004 !!
>Does anyone know difference between Epon done with (epoxy 218, NMA, 
>DDSA,
>DPM30) and Epon done with ( epoxy218, araldite 502, DDSA, DMP30) 
>wich one do you think is better for embedding thin human tissue ?
>thank you 
>Myriam
>Natural Implant
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>Histonet <@t> lists.utsouthwestern.edu
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>  
>

-- 
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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