[Histonet] Re: Histonet Digest, Vol 3, Issue 36

[Amos Brooks]

Alan,
         I've never really had a problem with that. I've seen problems 
similar to those described, however they were always caused by someone 
doing something like not using a tamper while embedding the tissue or if a 
sponge was used instead of a tissue or tea bag for processing. If the 
tissue is properly treated there will be no problem. I've seen 5-7 pieces 
in a block with no tissue loss even on recuts. The trick is to be REALLY 
careful. Don't believe everythnig you read.
Amos Brooks

At 12:00 PM 2/28/2004, you wrote:
>Message: 3
>Date: Fri, 27 Feb 2004 14:04:50 -0500
>From: ALAN MEEKER <ameeker <@t> mail.jhmi.edu>
>Subject: [Histonet] Prostate Needle Biopsies
>To: histonet <@t> lists.utsouthwestern.edu
>Message-ID: <3d67ee3db686.3db6863d67ee <@t> jhmimail.jhmi.edu>
>Content-Type: text/plain; charset=us-ascii
>
>Regarding the treatment of prostate needle biopsies, a paper recently 
>appeared in the journal UROLOGY addressing this question. Here is the 
>reference:
>
>Individual submission and embedding of prostate biopsies decreases rates 
>of equivocal pathology reports. Chakshu Gupta, Jian Z. Rena and Kirk J. Wojnoa
>
>Urology Vol. 63, No.1,  January 2004, pp. 83-86
>
>
>This is the Conclusion from the abstract:
>
>"Multiple needle biopsies submitted in 1 to 2 containers tend to entangle 
>and fragment and are difficult to embed in a single plane during 
>processing. The resulting loss of tissue surface area makes a definitive 
>diagnosis difficult on small foci of atypical glands, resulting in 
>equivocal pathology reports. The results of our study indicate that 
>individual submission and processing of prostate biopsies in 6 to 12 
>container kits reduces the monthly rates of equivocal diagnoses."
>
>-Alan Meeker