[Histonet] Advice.....secondary-biotinylated???

[Lab Histologia]

And the concentrated secondary-biotinylated antibodies??

-----Mensaje original-----
De: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] En nombre de Houston,
Ronnie Enviado el: Viernes, 27 de Febrero de 2004 12:12 p.m.
Para: 'Jackie.O'Connor <@t> abbott.com'; Ian Montgomery
CC: histonet <@t> lists.utsouthwestern.edu
Asunto: RE: [Histonet] Advice.



I think the important factor though,is that antibodies are aliquoted
into useable quantities to negate the freeze- thaw process affecting the
antibody's stability. Never had any problems with any antibodies that
have been frozen, including some >10 years old (before I get shot down,
these antibodies were for research purposes!)

Ronnie Houston
Director of Anatomic Pathology
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 287 7972
(804) 287 7906 - fax
ronnie_houston <@t> bshsi.com 




-----Original Message-----
From: Jackie.O'Connor <@t> abbott.com [mailto:Jackie.O'Connor <@t> abbott.com]
Sent: Friday, February 27, 2004 9:37 AM
To: Ian Montgomery
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Advice.


This would be a good time for the manufacturers to chime in.  When I 
worked where we manufactured antibodies (not all for IHC) we followed 
strict freeze/thaw guidelines for antibodies.  The maximum number of 
freeze thaw cycles for bulk antibodies was 3.  After that, the potency
of 
the antibodies deteriorated (per stability testing results).  The
potency 
of frozen as well as refrigerated antibodies was carefully monitored by 
pre-determined testing points to ensure reproducible testing results by 
customers.  The expiration date of refrigerated antibodies and other 
reagents was determined when the potency started to drop off.  The
potency 
of antibodies was tested up to a month after the determined expiration 
date, so we could ensure customers would continue to get satisfactory 
results right up to the expiration date. 

Jackie O'




Ian Montgomery <ian.montgomery <@t> bio.gla.ac.uk>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
02/27/2004 07:32 AM

 
        To:     histonet <@t> lists.utsouthwestern.edu
        cc: 
        Subject:        [Histonet] Advice.


         Antibody, store at 4C, do not freeze, stable for 1 year. What's

the current thinking. Are these antibodies affected by freezing? Or is
it 
just the company covering themselves against users thaw/freezing. In the

past I've just aliquoted and frozen anyway.
Ian.

Dr. Ian Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery <@t> bio.gla.ac.uk 


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