[Histonet] endogenous peroxidases in liver sections

[Patsy Ruegg]

Are you sure your staining is from endogenous peroxidase and not endogenous
biotin?  Are you using an avidin/biotin detection system?  there is a lot of
endogenous biotin in liver.  try and non biotin detection system and see if
the problem goes away.  I have not been able to block biotin in mouse liver
even with the most agressive and reported best a/b block reagents.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Cathy
Malcontenti-Wilson
Sent: Wednesday, February 25, 2004 3:36 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] endogenous peroxidases in liver sections


Hi,
Has anyone got any good method for blocking endogenous peroxidases in mouse
liver sections? We seem to have a lot of red and white blood cells and
plasma coming up in our negatives. We are using a Zymed MAX kit and SP kit
(mouse on mouse and universal kits). Some pearls of wisdom will be very
much appreciated.
Thanks
Cat


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