Fwd: [Histonet] Isopentane

Tue Feb 24 13:43:36 CST 2004

Charles and others - 
Did I read the original email wrong?  I thought the original author (I've 
lost track who it is - Atoska?) wanted to get rid of the isopentane and 
use LiN2 instead?
"due to the volatility"??

Jackie O'

"Charles Scouten" <cwscouten <@t> myneurolab.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
02/24/2004 12:04 PM

        To:     "Atoska S. Gentry" <gentras <@t> vetmed.auburn.edu>, "Histonet" 
<histonet <@t> pathology.swmed.edu>
        cc: 
        Subject:        RE: Re: Fwd: [Histonet] Isopentane

Isopentane can be chilled in a refrigeration device like the Clini RF, 
which also encloses it to prevent evaporation except when in use.  It is 
great for quick freezing tissue, does not require the hazard of liquid 
nitrogen, and does not freeze so fast it cracks the block.  See the 
following link for the Clini-RF

 http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=476401&catdesc=Histology+Equipment&CatThreeID=650&CatOneID=4&subcatdesc=Freezing+Devices&idsubcategory=187

Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 
www.myneurolab.com 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Atoska S. 
Gentry
Sent: Tuesday, February 24, 2004 11:24 AM
To: Histonet
Subject: Fwd: Re: Fwd: [Histonet] Isopentane

Hello, I'm not aware of a procedure for quick freezing in isopentane 
alone, we quick freeze by immerging the sample in isopentane chilled by 
liquid nitrogen. Please will you share with me your protocol for quick 
freezing in isopentane. Thanks, Atoska

>X-Mailer: Novell GroupWise Internet Agent 6.5.1
>Date:  Tue, 24 Feb 2004 10:50:58 -0600
>From: "Nita Searcy" <NSEARCY <@t> swmail.sw.org>
>To: <gentras <@t> vetmed.auburn.edu>
>Subject: Re: Fwd: [Histonet] Isopentane
>
>Isopentane is used to "quick freeze" frozen sections- can't liquid 
>nitrogen be use in its place? No specialty work (muscles, etc.) but 
>routine frozens.
>
> >>> "Atoska S. Gentry" <gentras <@t> vetmed.auburn.edu> 02/24/04 10:12AM
> >>>
>
>Hello, I don't believe isopentane can be substituted for liquid 
>nitrogen, they're usually used in conjunction. However, dry ice can be 
>substituted for liquid nitrogen. Best wishes. Atoska
>
> >X-Mailer: Novell GroupWise Internet Agent 6.5.1
> >Date: Tue, 24 Feb 2004 06:51:28 -0600
> >From: "Nita Searcy" <NSEARCY <@t> swmail.sw.org>
> >To: <histonet <@t> lists.utsouthwestern.edu>
> >X-Scan-Signature: 8550f81978efdbf6f5d10beda515049b
> >X-BeenThere: histonet <@t> lists.utsouthwestern.edu
> >X-Mailman-Version: 2.1.3
> >List-Id: For the exchange of information pertaining to 
> >histotechnology
>and
> >         related fields <histonet.lists.utsouthwestern.edu>
> >List-Unsubscribe:
> ><http://lists.utsouthwestern.edu/mailman/listinfo/histonet>,
> >
> ><mailto:histonet-request <@t> lists.utsouthwestern.edu?subject=unsubscribe
> >>
> >List-Archive: <http://lists.utsouthwestern.edu/pipermail/histonet>
> >List-Post: <mailto:histonet <@t> lists.utsouthwestern.edu>
> >List-Help:
><mailto:histonet-request <@t> lists.utsouthwestern.edu?subject=help>
> >List-Subscribe:
><http://lists.utsouthwestern.edu/mailman/listinfo/histonet>,
> >
><mailto:histonet-request <@t> lists.utsouthwestern.edu?subject=subscribe>
> >Sender: histonet-bounces <@t> lists.utsouthwestern.edu
> >X-Scan-Signature: 2f26e54c73378134ed276f0a1e9fcee8
> >X-SA-Exim-Mail-From: histonet-bounces <@t> lists.utsouthwestern.edu
> >Subject: [Histonet] Isopentane
> >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on
>swlx162.swmed.edu
> >X-Spam-Level:
> >X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no
>version=2.63
> >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003)
> >X-SA-Exim-Scanned: Yes
> >
> >For Vinny- or anyone. I am trying to substitute liquid nitrogen for 
> >Isopentane in the frozen lab due to volatility. Could not access 
> >Histologic article from May regarding laboratory accident. Anyone 
> >have any other incidents?  Help would be appreciated.
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet <@t> lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>Atoska S. Gentry B.S., HT(ASCP)
>Research Assistant III
>Scott-Ritchey Research Center
>College of Veterinary Medicine
>Auburn University, AL  36849
>Phone# (334)844-5579  Fax# (334)844-5850
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL  36849
Phone# (334)844-5579  Fax# (334)844-5850 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet