[Histonet] substance p

[Dana Settembre]

Hello Mike,
I have stained for Substance P using a company I had never used before;
The Arnel Products Co.
P. O. Box 20045/ New York, NY 10021 800-333-6078

I used it with DakoCytomation's ready to use Prot. K for 5 min. at room
temp.
The dilution I used was at 1:3000, using a human brain as a control.

Next time I might try Chemicon, as the other person on the Histonet
mentioned.

Good Luck
Dana Settembre
University Hospital - UMDNJ
Newark, NJ


>>> Mike Bromley <M.Bromley <@t> dgri.scot.nhs.uk> 2/24/2004 4:51:30 AM >>>
Hi All
Has anyone immunostained for substance P on formalin fixed paraffins.
I
would appreciate info re: antibody type and source, retrieval etc

Best Wishes

Mike Bromley

Chief Biomedical Scientist
Pathology
Dumfries & Galloway Royal Infirmary
Scotland, UK

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