Fwd: [Histonet] cutting frozen sections of myocardium

Atoska S. Gentry gentras <@t> vetmed.auburn.edu
Tue Feb 24 10:10:18 CST 2004


Hello is it possible for you to fix overnight or for 24hrs and then 
cryoprotect in sucrose/fixative or sucrose/PB before freezing for 
cryosectioning? If you know that fixation won't destroy the enzyme you 
might try this method. Best wishes. Atoska

>Date: Tue, 24 Feb 2004 10:16:44 +0000
>From: Shaumik Adhya <shaumik.adhya <@t> ucl.ac.uk>
>To: histonet <@t> lists.utsouthwestern.edu
>User-Agent: Internet Messaging Program (IMP) 3.2.1
>X-Originating-IP: 128.40.253.73
>X-UCL-MailScanner-Information: Please contact the UCL Helpdesk,
>         helpdesk <@t> ucl.ac.uk for more information
>X-UCL-MailScanner: Found to be clean
>X-Scan-Signature: 201832d891fa504721b2e650a5a52212
>X-BeenThere: histonet <@t> lists.utsouthwestern.edu
>X-Mailman-Version: 2.1.3
>List-Id: For the exchange of information pertaining to histotechnology and
>         related fields <histonet.lists.utsouthwestern.edu>
>List-Unsubscribe: 
><http://lists.utsouthwestern.edu/mailman/listinfo/histonet>,
> 
><mailto:histonet-request <@t> lists.utsouthwestern.edu?subject=unsubscribe>
>List-Archive: <http://lists.utsouthwestern.edu/pipermail/histonet>
>List-Post: <mailto:histonet <@t> lists.utsouthwestern.edu>
>List-Help: <mailto:histonet-request <@t> lists.utsouthwestern.edu?subject=help>
>List-Subscribe: <http://lists.utsouthwestern.edu/mailman/listinfo/histonet>,
>         <mailto:histonet-request <@t> lists.utsouthwestern.edu?subject=subscribe>
>Sender: histonet-bounces <@t> lists.utsouthwestern.edu
>X-Scan-Signature: 05628b3a735271e1629bc35bb8abda07
>X-SA-Exim-Mail-From: histonet-bounces <@t> lists.utsouthwestern.edu
>Subject: [Histonet] cutting frozen sections of myocardium
>X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on swlx162.swmed.edu
>X-Spam-Level:
>X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no version=2.63
>X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003)
>X-SA-Exim-Scanned: Yes
>
>Hi Histonetters,
>
>I'm a clinician trying to get to grips with histotechnology techniques for
>research.  I'm having a lot of difficulty cutting frozen sections of rat
>myocardium.  I seem to be cutting sections with a lot of holes in them, and
>the fibres seem poorly arrayed.  I'm cutting rat myocardium to practice 
>before
>human myocardial biopsies.
>
>The rats are killed, the hearts harvested, without fixation.  They're cut 
>into
>small pieces before staining for 20 hours in senescence associated beta
>galactosidase solution (essentially x-gal solution at pH 6).  They are then
>moutned into OCT, frozen in isopentane and cut on a cryotome at -20
>centigrade.  The slides are then fixed in 3% paraformaldehyde, wawshed in
>distilled water, before Haemotxylin & Eosin staining.
>
>Any advice, tips or tricks will be much appreciated.
>
>Shaumik Adhya
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL  36849
Phone# (334)844-5579  Fax# (334)844-5850 





More information about the Histonet mailing list