[Histonet] Cryostat sections

ames1 <@t> breathe.com ames1 <@t> breathe.com
Mon Feb 23 08:48:13 CST 2004


Hi all, 

I am experiencing problems with my cryostat sections. They keep floating off 
the slide when I am using antibody staining. I have tried removing the 
acetone for fixing, but that makes no difference. My slides are the same as 
I have always used and I was in the middle of a pack when this problem 
surfaced. I was wondering if anyone had any tips on how to keep the sections 
on the slide during staining. 

Thanks 

Amy Greenhalgh 

Scientist
Sequani Ltd
Bromyard Rd
Ledbury
HR8 1LH 






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